FHL2 Antibody (F4B2-B11)

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
F4B2-B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

FHL2 Antibody (F4B2-B11) Summary

Immunogen
The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FHL2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product works in Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Western Blot.
Publications
Read Publication using NBP1-51144.

Reactivity Notes

Human, Rat. May cross react with Mouse

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for FHL2 Antibody (F4B2-B11)

  • AAG11
  • down-regulated in rhabdomyosarcoma LIM protein
  • DRAL
  • DRALAAG11
  • FHL2
  • FHL-2
  • four and a half LIM domains 2
  • four and a half LIM domains protein 2
  • four and a half LIM-domain protein 2
  • LIM domain protein DRAL
  • Skeletal muscle LIM-protein 3
  • SLIM3
  • SLIM-3
  • SLIM3aging-associated gene 11

Background

The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine-rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl-1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FHL2 Antibody (NBP1-51144)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-51144 Applications Species
Koenig K. The role of Four-and-a-half LIM domain protein 2 in dendritic cell migration. 2012 Universitaet Bonn.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FHL2
Entrez