FHL1 Antibody

Western Blot: FHL1 Antibody [NBP2-16490] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FHL1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: FHL1 Antibody [NBP2-16490] - Mouse hind brain. FHL1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: FHL1 Antibody [NBP2-16490] - U87 xenograft, using FHL1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: FHL1 Antibody [NBP2-16490] - Mouse heart. FHL1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: FHL1 Antibody [NBP2-16490] - Mouse muscle. FHL1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Bv, Sh
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

FHL1 Antibody Summary

• Description: Novus Biologicals Rabbit FHL1 Antibody (NBP2-16490) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human FHL1. The exact sequence is proprietary.
• Localization: Isoform 1: Cytoplasm; Isoform 3: Cytoplasm; Nucleus; Isoform 2: Nucleus; Cytoplasm; cytosol
• Predicted Species: Rat (94%), Porcine (97%), Bovine (94%), Sheep (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FHL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for FHL1 Antibody

• bA535K18.1
• FHL1
• four and a half LIM domains 1
• HLH1
• HPLH1
• KYOT
• KYO-T
• LIM protein SLIMMER
• skeletal muscle LIM-protein1
• SLIM1
• SLIM1Isl-1 and Mec-3 domains 1
• SLIMMER
• XMPMA

Background

LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: FHL1
• Uniprot:
  • Q13642()