FHL1 Antibody (767418) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 767418
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

FHL1 Antibody (767418) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human FHL1
  Thr93-Asn166
  Accession # Q13642
• Specificity: Detects human FHL1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FHL1 Antibody (767418) [Alexa Fluor® 350]

• bA535K18.1
• FHL1
• four and a half LIM domains 1
• HLH1
• HPLH1
• KYOT
• KYO-T
• LIM protein SLIMMER
• skeletal muscle LIM-protein1
• SLIM1
• SLIM1Isl-1 and Mec-3 domains 1
• SLIMMER
• XMPMA

Background

FHL1 (Four-and-a-half LIM domains protein 1; also SLIM1 and KyoT1) is an approximately 35 kDa member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323 amino acids (aa) in length. It contains one C4-type Zn-finger region (aa 7-31), three distinct LIM domains (aa 40-77; 101-158; 162-212) and a bipartite NLS (aa 231-246). There is one 32 kDa splice variant that shows a 50 aa substitution for aa 231-323. This creates a fourth LIM domain. There are also alternative start sites 29 aa and 16 aa upstream of the standard site, a deletion of aa 168-296, and an 11 aa substitution for aa 81-91. Over aa 93-166, human FHL1 is 97% aa identical to mouse FHL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q13642()