FGFR3 Antibody

FGFR3-Antibody-Western-Blot-NB600-1509-img0001.jpg

Western Blot: FGFR3 Antibody [NB600-1509] - (A) Western blot analysis of apoptotic markers following 24 & 48 hrs of selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) treatment. (B) Western blot analysis of autophagic & ER stress markers, evaluated after 1, 3, 6 & 18 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM). (C) Acridine orange staining of cells after 12 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) (magnification 40 ×). (D) High-resolution transmission electron microscopy micrographs of control & 24 hrs Se-DL-cystine-treated cells, showing the presence of autophagosome, as evident from double-layered membrane structure with inclusion bodies. (E) Western blot detection of ubiquitinylated proteins after treatments with different selenium compounds (similar doses as previous) for 6 hrs. Five microgram cell lysate was loaded into each lane. The lane intensity was analysed by using Adobe Photoshop program (version CS5). beta -actin was used as a loading control (Fig. 4A & B). C: Control; 1: selenite; 2: GS-Se-SG; 3: Se-DL-cystine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24400844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FGFR3 Antibody Summary

• Immunogen: Synthetic peptide: KDLLPPAPPSSGGSRT, corresponding to amino acids 792-806 of the cytoplasmic region of Human FGFR3 with N-terminal added lysine.
• Localization: Plasma membrane; Single-pass type I membrane protein
• Specificity: Does not react with human FGFR1 or FGFR2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FGFR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Western Blot 1:1000

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FGFR3 Antibody

• CD333
• CEK
• EC 2.7.10
• FGF R3
• FGFR3
• fibroblast growth factor receptor 3
• HSFGFR3EX
• JTK4
• JTK4thanatophoric dwarfism

Background

Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation in a wide variety of cells of mesodermal, ectodermal and endodermal origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR3 Antibody (NB600-1509)(1)

Publication using NB600-1509

• Wallenberg M, Misra S, Wasik AM et al. Selenium induces a multi-targeted cell death process in addition to ROS formation. J Cell Mol Med 2014-01-08 [PMID: 24400844] (WB, Human)

Bioinformatics

• Gene Symbol: FGFR3
• Entrez:
  • 2261(Human)