FGF14 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

FGF14 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human FGF14.

  Source: E. coli

  Amino Acid Sequence: EPSLHDVGETVPKPGVTPSKSTSASAIMNGGKPVNKSKTT

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: FGF14
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-55745.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for FGF14 Recombinant Protein Antigen

• bA397O8.2
• FGF-14
• FHF4FHF-4
• fibroblast growth factor 14
• Fibroblast growth factor homologous factor 4
• SCA27MGC119129

Background

The FGF14 gene codes for a fibroblast growth factor 14 protein that in isoform 1 is 247 amino acids long at 27 kDA and in isoform 2 is 252 amino acids long at 28 kDA. This gene is a member of the fibroblast growth factor (FGF) family so is thought to participate in nervous system development and function. FGF14 is involved in regulation of actin cytoskeleton, MAPK signaling, mitochondrial apoptosis, paxillin pathways, and nuclear receptor activation by vitamin-A. It interacts with genes EGFR, FGFR1, FGFR2, FGFR3, and FGFR4. FGF14 is associated with pancreatitis, gingivitis, breast cancer, ladd syndrome, ataxia, cerebritis, retinitis, neuronitis, and adenocarcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FGF14