FGF acidic/FGF1 Antibody (OTI4D2)

Western Blot: FGF acidic/FGF1 Antibody (4D2) [NBP1-47748] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF1.

Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (4D2) [NBP1-47748] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGF1.

Western Blot: FGF acidic/FGF1 Antibody (4D2) [NBP1-47748] - FGF1 antibody ( 4D2 ) at 1:50000 dilution + recombinant Human FGF1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: OTI4D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

FGF acidic/FGF1 Antibody (OTI4D2) Summary

• Description: Novus Biologicals Mouse FGF acidic/FGF1 Antibody (OTI4D2) (NBP1-47748) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 16-155 of human AFGF (NP_000791) produced in E.coli.
• Specificity: This antibody is specific for Homo sapiens fibroblast growth factor 1 (acidic) (FGF1), transcript variant 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:5000-50000
• Theoretical MW: 15.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FGF acidic/FGF1 Antibody (OTI4D2)

• AFGF
• alpha
• alpha-ECGF
• beta-ECGF
• ECGF
• ECGFB
• ECGF-betaAcidic fibroblast growth factor
• endothelial cell growth factor, beta
• FGF acidic
• FGF-1
• FGFABeta-endothelial cell growth factor
• FGF-alpha
• fibroblast growth factor 1 (acidic)
• GLIO703
• HBGF1
• HBGF-1
• heparin-binding growth factor 1

Background

FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF1
• Entrez:
  • 2246(Human)