Recombinant Mouse FGF-9 Animal-Free Protein

SDS-Page: Recombinant Mouse FGF-9 Animal-Free Protein [NBP2-76181] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Mouse FGF-9 Animal-Free Protein [NBP2-76181] - FGF-9 Induced proliferation of 3T3 cells.

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Animal Free
• Concentration: LYOPH

Recombinant Mouse FGF-9 Animal-Free Protein Summary

• Description: An un-tagged bioactive recombinant protein corresponding to Mouse FGF-9
  
  

  Source: E. coli

  Amino Acid Sequence: MPLGEVGSYF GVQDAVPFGN VPVLPVDSPV LLNDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS

  Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

  Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage
  
  

  Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
• Specificity: FGF-9 is a monomer, 23.4 kDa (207 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: NR6R 3T3 proliferation, >1.0 x 10^5 units/mg (ED50 acceptance criteria are <10 ng/mL ).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF9
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 23.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Mouse FGF-9 Animal-Free Protein

• FGF9
• FGF-9
• fibroblast growth factor 9 (glia-activating factor)
• Fibroblast growth factor 9
• GAF
• glia-activating factor
• HBFG-9
• HBGF-9
• Heparin-binding growth factor 9
• MGC119914
• MGC119915
• SYNS3

Background

The protein encoded by the FGF9 gene is a member of the fibroblast growth factor (FGF) family. FGF family members possessbroad mitogenic and cell survival activities, and are involved in a variety of biological processes, includingembryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolatedas a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, thisprotein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homologof this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed amale-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF9