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273-F9) was serially diluted and captured by Mouse Anti-Human FGF‑9 Monoclonal Antibody (Catalog # MAB273) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-273-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
273-F9) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-9 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF-9 Monoclonal Antibody (Catalog # MAB273). The ND50 is typically 0.1-0.6 µg/mL." class="big_lightbox" target="_blank">
273-F9) was serially diluted and captured by Mouse Anti-Human FGF‑9 Monoclonal Antibody (Catalog # MAB273) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-273-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
273-F9) induces proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 1-5 ng/mL." class="big_lightbox" target="_blank">
The protein encoded by the FGF9 gene is a member of the fibroblast growth factor (FGF) family. FGF family members possessbroad mitogenic and cell survival activities, and are involved in a variety of biological processes, includingembryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolatedas a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, thisprotein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homologof this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed amale-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. (provided by RefSeq)
![Immunohistochemistry FGF-9 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/FGF-9_AF-273-NA_Immunohistochemistry_6657.jpg)
![N/A FGF-9 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_FGF9_DY273_ELISA_1881.jpg)
![Bioactivity FGF-9 [Unconjugated]](http://images.novusbio.com/images/protein/273-f9_recombinant-human-fgf-9-protein-bioactivity-2411202075619.jpg)
![Bioactivity FGF-9 [Unconjugated]](http://images.novusbio.com/fullsize/11233-f9_recombinant-human-fgf-9-hek293-expressed-protein-cf-bioactivity-2482022153910.jpg)
![ELISA FGF-9 Antibody [Unconjugated]](http://images.novusbio.com/images/af-273-na_human-fgf-9-affinity-purified-polyclonal-ab-elisa-639201852912304938.jpg)
![SDS-Page FGF-9 [Unconjugated]](http://images.novusbio.com/fullsize/11233-f9_recombinant-human-fgf-9-hek293-expressed-protein-cf-sds-page-1582022105750.jpg)
