FGF-9 Antibody (36912)

Recombinant Human FGF‑9 (Catalog # 273-F9) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑9 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF‑9 Monoclonal Antibody (Catalog # MAB273). The ND50 is typically 0.1‑0.6 µg/mL.

FGF-9 blockade reduces lung fibrocyte accumulation and fibrosis in TGF-beta 1 transgenic mice. TGF-beta 1 Tg+ and Tg− mice were administered 14 days of doxycycline and injected i.p. with either FGF-9 neutralizing antibody or isotype control on days—1, 2, 5, 8, and 11. (A) BAL leukocyte concentration (absolute # per μl). (B) Lung collagen content as measured by Sircol assay performed on the right lower lobe (RLL). (C) Trichrome staining of paraffin embedded lung sections from TGF-beta 1 Tg+ mice treated with isotype control (left) or anti-FGF-9 antibody (right). Scale bar = 100 microns (D,E) Percentages (D) and absolute number (E) of fibrocytes (CD45+ColI alpha 1+) in lung digests as determined by flow cytometry. (F) Quantity of Tregs (expressed as percentage of CD4 cells that are CD25+FoxP3+) in lung digests as determined by flow cytometry. n = 5 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24904415), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: B/N, ELISA(Cap)
• Clone: 36912
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FGF-9 Antibody (36912) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF-9
  Met1-Ser208
  Accession # P31371
• Specificity: Detects human FGF-9 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) FGF-4, 5, 6, 7, 16, rhFGF acidic, or rhFGF basic is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF9
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.1-0.6 ug/mL
• Application Notes: ELISA Detection: Human FGF-9 Biotinylated Antibody (Catalog number BAF273)
  Standard: Recombinant Human FGF-9 (Catalog number 273-F9)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-9 Antibody (36912)

• FGF9
• FGF-9
• fibroblast growth factor 9 (glia-activating factor)
• Fibroblast growth factor 9
• GAF
• glia-activating factor
• HBFG-9
• HBGF-9
• Heparin-binding growth factor 9
• MGC119914
• MGC119915
• SYNS3

Background

The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share 30‑50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.

The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.

1. Naruo, K. et al. (1993) J. Biol. Chem. 268:2857.
2. Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF-9 Antibody (MAB273)(6)

Publications using MAB273

• Haichuan H, Zofia P, Patricia H et al. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms. Cancer Cell. 10/7/2021 [PMID: 34624218]
• Haichuan H, Zofia P, Patricia H et al. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms. Cancer Cell. 2021-10-07 [PMID: 34624218]
• Sun C, Fukui H, Hara K, Zhang X, Kitayama Y, Eda H, Tomita T, Oshima T, Kikuchi S, Watari J, Sasako M, Miwa H FGF9 from cancer-associated fibroblasts is a possible mediator of invasion and anti-apoptosis of gastric cancer cells. BMC Cancer, 2015-04-30;15(0):333. 2015-04-30 [PMID: 25925261] (Neutralization, Human)
• Hendrix ND, Wu R, Kuick R, Schwartz DR, Fearon ER, Cho KR Fibroblast growth factor 9 has oncogenic activity and is a downstream target of Wnt signaling in ovarian endometrioid adenocarcinomas. Cancer Res., 2006-02-01;66(3):1354-62. 2006-02-01 [PMID: 16452189] (Western Blot, Human)
• Cinaroglu A, Ozmen Y, Ozdemir A, Ozcan F, Ergorul C, Cayirlioglu P, Hicks D, Bugra K Expression and possible function of fibroblast growth factor 9 (FGF9) and its cognate receptors FGFR2 and FGFR3 in postnatal and adult retina. J. Neurosci. Res., 2005-02-01;79(3):329-39. 2005-02-01 [PMID: 15614790] (IHC-P, Neutralization, Rat)
• Schmahl J, Kim Y, Colvin JS, Ornitz DM, Capel B Fgf9 induces proliferation and nuclear localization of FGFR2 in Sertoli precursors during male sex determination. Development, 2004-06-30;131(15):3627-36. 2004-06-30 [PMID: 15229180] (IHC, Human)

Bioinformatics

• Gene Symbol: FGF9
• Uniprot:
  • P31371()