FGF-9 Antibody (36912)

Images

 
Recombinant Human FGF‑9 (Catalog # 273-F9) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑9 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
36912
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

FGF-9 Antibody (36912) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF-9
Met1-Ser208
Accession # P31371
Specificity
Detects human FGF-9 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) FGF-4, 5, 6, 7, 16, rhFGF acidic, or rhFGF basic is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FGF9
Purity
Protein A or G purified from ascites
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.1-0.6 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human FGF-9 Biotinylated Antibody (Catalog number BAF273)
Standard: Recombinant Human FGF-9 (Catalog number 273-F9)
Publications
Read Publications using
MAB273 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-9 Antibody (36912)

  • FGF9
  • FGF-9
  • fibroblast growth factor 9 (glia-activating factor)
  • Fibroblast growth factor 9
  • GAF
  • glia-activating factor
  • HBFG-9
  • HBGF-9
  • Heparin-binding growth factor 9
  • MGC119914
  • MGC119915
  • SYNS3

Background

The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share 30‑50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.

The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.

  1. Naruo, K. et al. (1993) J. Biol. Chem. 268:2857.
  2. Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF-9 Antibody (MAB273)(4)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: IHC, Neutralization, Western Blot.


Filter By Application
IHC
(1)
Neutralization
(2)
Western Blot
(1)
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Filter By Species
Human
(3)
Rat
(1)
All Species

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Bioinformatics

Gene Symbol FGF9
Uniprot