Fetuin A/AHSG Antibody

Western blot shows lysates of rat liver tissue and rat lung tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Rat Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7510) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fetuin A/AHSG at approximately 59-64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Fetuin A/AHSG Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Fetuin A/AHSG
  Ala19-Ile352
  Accession # P24090
• Specificity: Detects rat Fetuin A/AHSG in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Fetuin A is observed, and less than 3% cross-reactivity with recombinant human Fetuin A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AHSG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fetuin A/AHSG Antibody

• A2HS
• AHS
• AHSG
• alpha-2-HS-glycoprotein
• alpha-2-Z-globulin
• Ba-alpha-2-glycoprotein
• FETUAba-alpha-2-glycoprotein
• Fetuin A
• fetuin-A
• HSGA

Background

Fetuin A (from the Latin word fetus for "offspring"; also alpha 2-HS [Heremans and Schmid] glycoprotein/AHSG, 59 kDa BSP and pp63) is a 59-63 kDa soluble, highly glycosylated type 3 member of the fetuin family of proteins. Originally isolated from fetal calf serum, rat fetuin A is now reported to be secreted by hepatocytes and, possibly, osteoblasts. Functionally, fetuin A appears to complex with matrix Gla protein, bind calcium phosphate and to regulate matrix mineralization. While it does not dissolve existing mineral, it does inhibit unwarranted tissue mineralization and the inflammatory reaction that accompanies it. A role for fetuin A in the regulation of insulin receptor signaling is unclear. Mature rat fetuin A is 334 amino acids (aa) in length. It contains two cystatin fetuin A type I domains (aa 19-133 and 144-250) that control mineralization, and a C-terminal domain (aa 255-352) that may interact with the insulin receptor. There are at least four utilized phosphorylation sites at Ser138, 313, 316 and 318, and one potential internal cleavage site between Arg143-Lys144 that, if utilized, would generate a disulfide-linked heterodimer. Mature rat fetuin A shares 85% and 61% aa sequence identity with mouse and human fetuin A, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AHSG
• Uniprot:
  • P24090()