Fetuin A/AHSG Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Fetuin A/AHSG Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Fetuin A/AHSG (R&D Systems, Catalog # 1563-PI)
  Ala19-Ile345
  Accession # NP_038493
• Specificity: Detects mouse Fetuin A/AHSG in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human Fetuin A/AHSG is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AHSG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fetuin A/AHSG Antibody

• A2HS
• AHS
• AHSG
• alpha-2-HS-glycoprotein
• alpha-2-Z-globulin
• Ba-alpha-2-glycoprotein
• FETUAba-alpha-2-glycoprotein
• Fetuin A
• fetuin-A
• HSGA

Background

Mouse Fetuin A, also known as alpha ₂-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It has been also called "countertrypsin" because of its ability to inhibit trypsin (1). It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (2, 3). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (4). The major form of plasma Fetuin A corresponds to two disulfide bond-linked chains derived from the single chain (5). Fetuin-A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (7, 8).

1. Yamamoto, K. and H. Sinohara (1993) J. Biol. Chem. 268:17750.
2. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
3. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
4. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
5. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta 671:78.
6. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
7. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
8. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fetuin A/AHSG Antibody (AF1563)(2)

Publications using AF1563

• V Kothari, JR Babu, ST Mathews AMP activated kinase negatively regulates hepatic Fetuin-A via p38 MAPK-C/EBPbeta/E3 Ubiquitin Ligase Signaling pathway PLoS ONE, 2022-05-06;17(5):e0266472. 2022-05-06 [PMID: 35522655] (Immunoprecipitation, Western Blot, Human)
• VK Harris, L Bell, RA Langan, J Tuddenham, M Landy, SA Sadiq Fetuin-A deficiency protects mice from Experimental Autoimmune Encephalomyelitis (EAE) and correlates with altered innate immune response PLoS ONE, 2017-04-07;12(4):e0175575. 2017-04-07 [PMID: 28388685] (IHC-P, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: AHSG
• Uniprot:
  • NP_038493()