Fc gamma RIIB/CD32b Antibody

Western Blot: Fc gamma RIIB/CD32b Antibody [NBP1-69757] - Fc gamma RIIB/CD32b antibody (1g/ml) staining of Daudi cell lysate (RIPA buffer, (35g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody [NBP1-69757] - Analysis of anti-CD32B antibody with human liver at concentration 3.75 ug/ml.

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody [NBP1-69757] - Analysis of anti-CD32B antibody with human placenta at concentration 3.75 ug/ml.

Product Discontinued

Fc gamma RIIB/CD32b Antibody Summary

• Immunogen: Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus of the Fc gamma RIIB/CD32b protein sequence according to NP_003992.3, NP_001002273.1, NP_001002274.1, NP_001002275.1, and NP_001177757.1.
• Specificity: Human Fc gamma RIIB/CD32b. This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1).
• Predicted Species: Primate (100%), Monkey (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FCGR2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA 1:32000
  • Western Blot 0.3-1 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for Fc gamma RIIB/CD32b Antibody

• CD32 antigen
• CD32b
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RIIB
• FCG2
• Fc-gamma RII-b
• fc-gamma-RIIb
• FCGR2
• FCGR2B
• FcgRIIB
• FCRIIB
• fcRII-b
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR2B
• Entrez:
  • 2213(Human)
• Uniprot:
  • P31994()