Fc gamma RIIB/CD32b Antibody Pair [HRP]

Sandwich ELISA: Fc gamma RIIB/CD32b Antibody Pair [HRP] [NBP2-79669] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP
• Standard Curve Range: 31.25-2000 pg/ml (example only; lot dependent)
• Sensitivity: 31.25 pg/ml (example only; lot dependent)

Fc gamma RIIB/CD32b Antibody Pair [HRP] Summary

• Description: Solid Phase sandwich ELISA for the quantitative determination of Human Fc gamma RIIB/CD32b.
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Fc gamma RIIB/CD32b.
• Standard Curve Range: 31.25-2000 pg/ml (example only; lot dependent)
• Sensitivity: 31.25 pg/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: FCGR2B

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Fc gamma RIIB/CD32b Antibody Pair [HRP]

• CD32 antigen
• CD32b
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RIIB
• FCG2
• Fc-gamma RII-b
• fc-gamma-RIIb
• FCGR2
• FCGR2B
• FcgRIIB
• FCRIIB
• fcRII-b
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: FCGR2B