Fc epsilon RI Antibody (1F2A9) - BSA Free

Western Blot: Fc epsilon RI Antibody (1F2A9) [NBP2-37402] - Western blot analysis using FCER1A mouse mAb against SW620 (1), A549 (2), and A431 (3) cell lysate.

Flow Cytometry: Fc epsilon RI Antibody (1F2A9) [NBP2-37402] - Flow cytometric analysis of HEK293 cells using FCER1A mouse mAb (green) and negative control (red).

Western Blot: Fc epsilon RI Antibody (1F2A9) [NBP2-37402] - Western blot analysis using FCER1A mAb against human FCER1A recombinant protein. (Expected MW is 32.5 kDa)

ELISA: Fc epsilon RI Antibody (1F2A9) [NBP2-37402] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 1F2A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Fc epsilon RI Antibody (1F2A9) - BSA Free Summary

• Description: Novus Biologicals Mouse Fc epsilon RI Antibody (1F2A9) - BSA Free (NBP2-37402) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Fc epsilon RI (AA: 42-103) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCER1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 29.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Fc epsilon RI Antibody (1F2A9) - BSA Free

• alpha polypeptide
• Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Fc-epsilon RI-alpha
• FcERI
• high affinity immunoglobulin epsilon receptor alpha-subunit
• high affinity immunoglobulin epsilon receptor subunit alpha
• high-affinity, of mast cells, alpha polypeptide

Background

The allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FceRI receptor is a tetrameric complex, consisting of a a-chain, b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCER1A