FBXW5 Antibody - BSA Free

Immunohistochemistry: FBXW5 Antibody - BSA Free [NBP3-46328] - Immunohistochemistry of paraffin-embedded human kidney tissue slide using (FBXW5 Antibody) at dilution of 1:50

Western Blot: FBXW5 Antibody - BSA Free [NBP3-46328] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (FBXW5 Antibody) at dilution of 1:600

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FBXW5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FBXW5 Antibody - BSA Free (NBP3-46328) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: F-box and WD repeat domain containing 5. (Uniprot# Q969U6)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXW5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for FBXW5 Antibody - BSA Free

• DKFZp434B205
• F-box and WD repeat domain containing 5
• F-box and WD-40 domain protein 5
• F-box and WD-40 domain-containing protein 5
• F-box/WD repeat-containing protein 5
• Fbw5
• MGC20962
• RP11-229P13.10
• WD repeat-containing F-box protein FBW5

Background

The FBXW5 gene encodes a F-box/WD repeat-containing protein 5 that exists in two isoforms. Isoform 1 is 556 amino acids long, at nearly 64 kDA while isoform 2 is 377 amino acids long at 42 kDA. This protein encoded by this gene functions in substrate recognition of SCF as well as DCX E3 ubiquitin-protein ligase complexes. It may also work as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B signaling pathway. FBXW5 participates in protein chaperonin-mediated protein folding and metabolism as well as the association of TriC/CCT with target proteins during biosynthesis. It is known to interact with genes CUL1, MDFI, SKP1, KRTAP4-12, and UBE2R2. FBXW5 is associated with tuberous sclerosis as well as hepatitis b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBXW5