FBXW11 Overexpression Lysate (Native)

Images

 
Western Blot: FBXW11 Overexpression Lysate (Adult Normal) [NBL1-10644] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FBXW11.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FBXW11 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204407 and based on accession number NM_033644. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens F-box and WD repeat domain containing 11 (FBXW11), transcript variant 2, mRNA.
Gene
FBXW11

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
60.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FBXW11

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FBXW11 Overexpression Lysate (Native)

  • beta-transducin repeat-containing protein 2
  • BTRC2F-box/WD repeat-containing protein 1B
  • BTRCP2FBW1B
  • F-box and WD repeat domain containing 11
  • F-box and WD-40 domain protein 11
  • F-box and WD-40 domain protein 1B
  • F-box protein Fbw1b
  • F-box/WD repeat-containing protein 11
  • Fbw11
  • FBXW1BFbw1b
  • Homologous to Slimb protein
  • Hos
  • KIAA0696F-box and WD repeats protein beta-TrCP2

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 3. This variant encodes a shorter isoform (B), compared to isoform C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FBXW11