FBXO42 Antibody (OTI2G7) [DyLight 650]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI2G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

FBXO42 Antibody (OTI2G7) [DyLight 650] Summary

• Immunogen: Recombinant protein fragment corresponding to amino acids 356-717 of human FBXO42 (NP_061867) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBXO42
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for FBXO42 Antibody (OTI2G7) [DyLight 650]

• F-box only protein 42
• F-box protein 42
• Fbx42
• JFK
• Just one F-box and Kelch domain-containing protein
• KIAA1332FBX42

Background

F-box only protein 42 (FBXO42) is a substrate recognition component of the SKP1-CUL1-F-box protein (SCF) type E3 ubiquitin ligase complex. The FBXO42 protein recognizes p53/TP53; which promotes its ubiquitination and degradation. FBXO42 is a member of the F-box protein family. Members of this family are characterized by an approximately 40-amino acid F-box motif. F-box proteins are also known to interact with SKP1 and other ubiquitination targets through their interaction domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBXO42