EFHD2 Antibody

Images

 
Western Blot: EFHD2 Antibody [NB100-1477] - Staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477] - Analysis of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

EFHD2 Antibody Summary

Immunogen
Peptide with sequence CAAFKELQSTFK corresponding to C-Terminus according to NP_077305.2.
Specificity
This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EFHD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 10 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 30 kDa band observed in human tonsil, thymus and lymph node and in mouse thymus and lymph node lysates (calculated MW of 26.7 kDa band according to NP_077305.2). IHC-P: Human tonsil shows membranous staining in selected cells.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PADI4 Lysate (NBL1-14061)
Reviewed Applications
Read 1 Review rated 5
using
NB100-1477 in the following applications:

Publications
Read Publications using
NB100-1477 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine, Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EFHD2 Antibody

  • EF hand domain containing 2
  • EF hand domain family, member D2
  • EF-hand domain family, member D2
  • EF-hand domain-containing protein D2
  • MGC4342
  • swiprosin 1
  • swiprosin-1
  • SWS1

Background

Found in lymphocytes; preferentially expressed in CD8+ cells. Contains 2 EF Hand domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EFHD2 Antibody (NB100-1477)(3)

Review for EFHD2 Antibody (NB100-1477) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: mice.

Reviews using NB100-1477:
Filter by Applications
WB
(1)
All Applications
Filter by Species
mice
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB mice 04/27/2017
View

Summary

ApplicationWestern Blot
Sample Testedmice
Speciesmice

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for EFHD2 Antibody (NB100-1477) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on EFHD2

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
04/27/2017
Application: WB
Species: mice

Bioinformatics

Gene Symbol EFHD2
Entrez
Uniprot