FBXO42 Antibody (OTI1H4)

Western Blot: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO42.

Immunohistochemistry: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of Human pancreas tissue.

Flow Cytometry: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by FBXO42 antibody.

Immunohistochemistry: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of Human Kidney tissue.

Immunohistochemistry: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of Human prostate tissue.

Immunohistochemistry: FBXO42 Antibody (1H4) [NBP2-45621] - Analysis of Carcinoma of Human prostate tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

FBXO42 Antibody (OTI1H4) Summary

• Description: Novus Biologicals Mouse FBXO42 Antibody (OTI1H4) (NBP2-45621) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 356-717 of human FBXO42 (NP_061867) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBXO42
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 77.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FBXO42 Antibody (OTI1H4)

• F-box only protein 42
• F-box protein 42
• Fbx42
• JFK
• Just one F-box and Kelch domain-containing protein
• KIAA1332FBX42

Background

F-box only protein 42 (FBXO42) is a substrate recognition component of the SKP1-CUL1-F-box protein (SCF) type E3 ubiquitin ligase complex. The FBXO42 protein recognizes p53/TP53; which promotes its ubiquitination and degradation. FBXO42 is a member of the F-box protein family. Members of this family are characterized by an approximately 40-amino acid F-box motif. F-box proteins are also known to interact with SKP1 and other ubiquitination targets through their interaction domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBXO42