FBXO31 Antibody - BSA Free

Western Blot: FBXO31 Antibody [NBP1-19088] - Detection of Human and Mouse FBXO31 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FBXO31 antibody NBP1-19088 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FBXO31 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 489 and 539 of human F-box protein 31 using the numbering given in entry NP_079011.3 (GeneID 79791).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXO31
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID 24623383)
  • Immunoprecipitation Negative
  • Western Blot 1:1000-1:5000
• Theoretical MW: 60.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FBXO31 Antibody - BSA Free

• DKFZp434B027
• F-box only protein 31
• F-box protein 31
• FBX14DKFZp434J1815
• Fbx31
• FBXO14FLJ22477
• MGC15419
• MGC9527
• pp2386
• putative breast cancer tumor-suppressor
• SCF ubiquitin ligase specificity factor

Background

Members of the F-box protein family, such as FBXO31, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXO31 Antibody (NBP1-19088)(3)

Publications using NBP1-19088

• Duan S, Moro L, Qu R Et al. Loss of FBXO31-mediated degradation of DUSP6 dysregulates ERK and PI3K-AKT signaling and promotes prostate tumorigenesis Cell reports 2021-10-19 [PMID: 34686346] (WB, IF/IHC, Human)
• Mir A, Sritharan K, Mittal K et al. Truncation of the E3 ubiquitin ligase component FBXO31 causes non-syndromic autosomal recessive intellectual disability in a Pakistani family. Hum. Genet. 2014-03-13 [PMID: 24623383] (ICC/IF, Human)
• Duan S, Cermak L, Pagan JK et al. FBXO11 targets BCL6 for degradation and is inactivated in diffuse large B-cell lymphomas. Nature 2012-01-01 [PMID: 22113614]

Bioinformatics

• Gene Symbol: FBXO31
• Entrez:
  • 79791(Human)
  • 76454(Mouse)
• Uniprot:
  • Q5XUX0(Human)