FBXL20 Antibody - BSA Free

Images

 
Western Blot: FBXL20 Antibody [NBP1-77317] - A20 cell lysate with FBXL20 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry-Paraffin: FBXL20 Antibody [NBP1-77317] - Human brain tissue using FBXL20 antibody at 2.5 ug/ml.
Immunofluorescence: FBXL20 Antibody [NBP1-77317] - Immunofluorescence of scrapper in human brain tissue with scrapper antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

FBXL20 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human SCRAPPER. The immunogen is located within the first 50 amino acids of SCRAPPER. Amino Acid Squence: SDEAVINKKLPKELLLR
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBXL20
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
Control Peptide
FBXL20 Peptide (NBP1-77317PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for FBXL20 Antibody - BSA Free

  • DKFZp564O2364
  • Fbl2
  • Fbl20
  • F-box and leucine-rich repeat protein 20FLJ21037
  • F-box protein FBL2
  • F-box/LRR-repeat protein 20
  • F-box/LRR-repeat protein 2-like
  • MGC15482

Background

Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FBXL20
Uniprot