FBXL2 Antibody - BSA Free

Immunohistochemistry-Paraffin: FBXL2 Antibody [NBP1-33641] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.

Immunocytochemistry/ Immunofluorescence: FBXL2 Antibody [NBP1-33641] - FBXL2 antibody [N1], N-term detects FBXL2 protein at nucleolus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FBXL2 stained by FBXL2 antibody [N1], N-term (NBP1-33641) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

Western Blot: FBXL2 Antibody [NBP1-33641] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FBXL2 antibody [N1], N-term (NBP1-33641) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: FBXL2 Antibody [NBP1-33641] - FBXL2 antibody [N1], N-term detects FBXL2 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.FBXL2 stained by FBXL2 antibody [N1], N-term (NBP1-33641) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FBXL2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FBXL2 Antibody - BSA Free (NBP1-33641) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human FBXL2. The exact sequence is proprietary.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXL2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for FBXL2 Antibody - BSA Free

• FBL2FBL3F-box protein containing leucine-rich repeats
• F-box and leucine-rich repeat protein 2DKFZp564P0622
• F-box protein FBL2/FBL3
• F-box/LRR-repeat protein 2

Background

FBXL2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: FBXL2