Recombinant Human FBP2 His Protein

SDS-Page: FBP2 Recombinant Protein [NBP2-52235] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human FBP2 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-339 of Human FBP2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTDRSPF ETDMLTLTRY VMEKGRQAKG TGELTQLLNS MLTAIKAISS AVRKAGLAHL YGIAGSVNVT GDEVKKLDVL SNSLVINMVQ SSYSTCVLVS EENKDAIITA KEKRGKYVVC FDPLDGSSNI DCLASIGTIF AIYRKTSEDE PSEKDALQCG RNIVAAGYAL YGSATLVALS TGQGVDLFML DPALGEFVLV EKDVKIKKKG KIYSLNEGYA KYFDAATTEY VQKKKFPEDG SAPYGARYVG SMVADVHRTL VYGGIFLYPA NQKSPKGKLR LLYECNPVAY IIEQAGGLAT TGTQPVLDVK PEAIHQRVPL ILGSPEDVQE YLTCVQKNQA GS

• Details of Functionality: Specific activity is > 1,500 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FBP2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human FBP2 His Protein

• D-fructose-1,6-bisphosphate 1-phosphohydrolase 2
• EC 3.1.3
• EC 3.1.3.11
• FBPase 2
• fructose-1,6-bisphosphatase 2
• fructose-1,6-bisphosphatase isozyme 2
• hexosediphosphatase
• MGC142192
• muscle fructose-bisphosphatase

Background

FBP2 encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FBP2