Fascin Antibody (rFSCN1/6464)

IHC analysis of formalin-fixed, paraffin-embedded human kidney. Cytoplasmic staining using Fascin Antibody (rFSCN1/6464) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human pancreas. Cytoplasmic staining using Fascin Antibody (rFSCN1/6464) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: rFSCN1/6464
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Fascin Antibody (rFSCN1/6464) Summary

• Description: Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length recombinant human FSCN1 protein (Uniprot: Q16658)
• Localization: Cell cortex, Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Cytosol, Filopodium, Invadopodium, Microvillus, Stress fiber
• Source: CHO
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FSCN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Fascin Antibody (rFSCN1/6464)

• FAN1
• FAN1,55 kDa actin-bundling protein
• fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Fascin
• FLJ38511
• FSCN1
• HSN
• p55actin bundling protein
• singed (Drosophila)-like (sea urchin fascin homolog like)
• singed-like (fascin homolog, sea urchin)
• Singed-like Protein
• SNL
• SNLfascin

Background

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FSCN1