Fascin Antibody (FSCN1/418) [HRP]

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
• Clone: FSCN1/418
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

Fascin Antibody (FSCN1/418) [HRP] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Full length recombinant human Fascin protein (Uniprot: Q16658)
• Localization: Cytoplasmic
• Marker: Reed-Sternberg Cell Marker
• Specificity: Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkins disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FSCN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-47802. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for Fascin Antibody (FSCN1/418) [HRP]

• FAN1
• FAN1,55 kDa actin-bundling protein
• fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Fascin
• FLJ38511
• FSCN1
• HSN
• p55actin bundling protein
• singed (Drosophila)-like (sea urchin fascin homolog like)
• singed-like (fascin homolog, sea urchin)
• Singed-like Protein
• SNL
• SNLfascin

Background

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FSCN1