FAM65B Antibody (2F6-1A11) - Azide and BSA Free

Western Blot: FAM65B Antibody (2F6-1A11) [H00009750-M02] - Analysis of C6orf32 expression in transfected 293T cell line by C6orf32 monoclonal antibody (M02), clone 2F6-1A11.Lane 1: C6orf32 transfected lysate(66 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: FAM65B Antibody (2F6-1A11) [H00009750-M02] - Detection limit for recombinant GST tagged FAM65B is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 2F6-1A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FAM65B Antibody (2F6-1A11) - Azide and BSA Free Summary

• Immunogen: C6orf32 (AAH01232.1, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLVGSQSFSPGGPNGIIRSQSFAGFSGLQERRSRCNSFIENSSALKKPQAKLKKMHNLGHKNNNPPKEPQPKRVEEVYRALKNGLDEYLEVHQTELDKLTAQLKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEAYCIQRRLQDGASKMKQAFATSPASKAARESLTEINRSFKEYTENMCTIEVELENLLGEFSIKMKGLAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGKQSWDGEETVFLPLIVGFISIKVTELKGLATHILVGSVTCETKELFAARPQVVAVDINDLGTIKLNLEITWYPFDVEDMTASSGAGNKAAALQRRMSMYSQGTPETPTFKDHSFFSNLPDDIFENGKAAEEKMPLSLSFSDLPNGDCALTSHSTGSPSNSTNPEITITPAEFNLSSLASQNEGMDDTSSASSRNSLGEGQEPKSHLKEEDPEEPRKPASAPSEACRRQSSGAGAEHLFLENDVAEALLQESEEASELKPVELDTSEGNITKQLVKRLTSAEVPMATDRLLSEGSVGGESEGCRSFLDGSLEDAFNGLLLALEPHKEQYKEFQDLNQEVMNLDDILKK
• Specificity: C6orf32 - chromosome 6 open reading frame 32
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIPOR2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FAM65B Antibody (2F6-1A11) - Azide and BSA Free

• C6orf32
• DIFF48DIFF40
• family with sequence similarity 65, member B
• PL48KIAA0386chromosome 6 open reading frame 32

Background

The protein encoded by this gene stimulates the formation of a non-mitotic multinucleate syncytium from proliferative cytotrophoblasts during trophoblast differentiation. Two alternatively spliced transcript variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FAM65B Antibody (H00009750-M02)(1)

Publication using H00009750-M02

• Rougerie P, Largeteau Q, Megrelis L et al. Fam65b Is a New Transcriptional Target of FOXO1 That Regulates RhoA Signaling for T Lymphocyte Migration. J Immunol. 2012-12-14 [PMID: 23241886]

Bioinformatics

• Gene Symbol: RIPOR2
• Entrez:
  • 9750(Human)
• Uniprot:
  • AAH01232(Human)