FAM65B Antibody (2E6-1B10) - Azide and BSA Free

Sandwich ELISA: FAM65B Antibody (2E6-1B10) [H00009750-M01] - Detection limit for recombinant GST tagged FAM65B is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 2E6-1B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FAM65B Antibody (2E6-1B10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse FAM65B Antibody (2E6-1B10) - Azide and BSA Free (H00009750-M01) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-FAM65B Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: C6orf32 (AAH01232.1, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLVGSQSFSPGGPNGIIRSQSFAGFSGLQERRSRCNSFIENSSALKKPQAKLKKMHNLGHKNNNPPKEPQPKRVEEVYRALKNGLDEYLEVHQTELDKLTAQLKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEAYCIQRRLQDGASKMKQAFATSPASKAARESLTEINRSFKEYTENMCTIEVELENLLGEFSIKMKGLAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGKQSWDGEETVFLPLIVGFISIKVTELKGLATHILVGSVTCETKELFAARPQVVAVDINDLGTIKLNLEITWYPFDVEDMTASSGAGNKAAALQRRMSMYSQGTPETPTFKDHSFFSNLPDDIFENGKAAEEKMPLSLSFSDLPNGDCALTSHSTGSPSNSTNPEITITPAEFNLSSLASQNEGMDDTSSASSRNSLGEGQEPKSHLKEEDPEEPRKPASAPSEACRRQSSGAGAEHLFLENDVAEALLQESEEASELKPVELDTSEGNITKQLVKRLTSAEVPMATDRLLSEGSVGGESEGCRSFLDGSLEDAFNGLLLALEPHKEQYKEFQDLNQEVMNLDDILKK
• Specificity: C6orf32 - chromosome 6 open reading frame 32
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIPOR2
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:100-1:2000
• Application Notes: Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence and Immunoprecipitation reported in scientific literature (PMID 24687993)

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FAM65B Antibody (2E6-1B10) - Azide and BSA Free

• C6orf32
• DIFF48DIFF40
• family with sequence similarity 65, member B
• PL48KIAA0386chromosome 6 open reading frame 32

Background

The protein encoded by this gene stimulates the formation of a non-mitotic multinucleate syncytium from proliferative cytotrophoblasts during trophoblast differentiation. Two alternatively spliced transcript variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FAM65B Antibody (H00009750-M01)(3)

Publications using H00009750-M01

• Ishiba R, Santos A, Almeida C et al. Faster regeneration associated to high expression of Fam65b and Hdac6 in dysferlin-deficient mouse. J Mol Histol. 2019-06-19 [PMID: 31218594]
• Balasubramanian A, Kawahara G, Gupta VA et al. Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation. FASEB J. 2014-03-31 [PMID: 24687993] (WB, ICC/IF, IP, Human)
• Yoon S, Molloy MJ, Wu MP et al. C6ORF32 is upregulated during muscle cell differentiation and induces the formation of cellular filopodia. Dev Biol 2007-01-01 [PMID: 17150207]

Bioinformatics

• Gene Symbol: RIPOR2
• Entrez:
  • 9750(Human)
• Uniprot:
  • AAH01232(Human)