FAM120A Antibody - BSA Free

Western Blot: FAM120A Antibody [NBP1-77367] - SK-N-SH cell lysate with FAM120A antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: FAM120A Antibody - BSA Free [NBP1-77367] - Immunofluorescence of FAM120A in Rat Brain cells with FAM120A antibody at 20 u/mL.

Immunohistochemistry: FAM120A Antibody - BSA Free [NBP1-77367] - Immunohistochemistry of FAM120A in rat brain tissue with FAM120A antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FAM120A Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide from near the center of human FAM120A. The immunogen is located within amino acids 610 - 660 of FAM120A. Amino Acid Squence: SRKKTERLAFRKNRLPPE
• Specificity: This antibody will not cross-react with FAM120B / PGCC1.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAM120A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  FAM120A Peptide (NBP1-77367PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FAM120A Antibody - BSA Free

• C9orf10MGC133257
• chromosome 9 open reading frame 10
• constitutive coactivator of PPAR-gamma-like protein 1
• DNA polymerase-transactivated protein 1
• DNA polymerase-transactivated protein 5
• DNAPTP1
• DNAPTP5
• family with sequence similarity 120A
• KIAA0183MGC111527
• OSSA
• Oxidative stress-associated Src activator
• Protein FAM120A

Background

FAM120A (C9orf10) is a member of the constitutive coactivator of PPAR gamma family and the gene was mapped to chromosome 9q22.31. FAM120A was recently detected within the Pur-alpha-containing mRNA-protein complex in the brain. As a novel RNA-binding protein, FAM120A is a critical component of the oxidative stress-induced survival signaling. It may participate in mRNA transport in the cytoplasm. FAM120A activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. FAM120A protects cells from apoptosis through activation of SRKs in response to oxidative stress. Blocking of the survival signaling mediated by FAM120A, which sensitizes the cancer cells to stress-induced apoptosis, may be a novel therapeutic approach for gastric scirrhous carcinoma cells. This antibody will not cross-reacti with FAM120B / PGCC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAM120A
• Uniprot:
  • NP_055427(Human)