CDCP1 Antibody (309137)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 309137
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CDCP1 Antibody (309137) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
  Ala33-Leu666
  Accession # NP_073753
• Specificity: Detects human CDCP1 in direct ELISAs and Western blots. Recognizes an epitope found between aa 33‑369 of human CDCP1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDCP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDCP1 Antibody (309137)

• CD318 antigen
• CD318
• CDCP1
• CUB domain containing protein 1
• CUB domain-containing protein 1
• Membrane glycoprotein gp140
• SIMA135
• SIMA135TRASK
• Subtractive immunization M plus HEp3-associated 135 kDa protein
• Transmembrane and associated with src kinases

Background

CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDCP1 Antibody (MAB2666)(5)

Publications using MAB2666

• Iwata M, Torok-Storb B, Wayner E, Carter W CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production. PLoS ONE, 2014-10-02;9(10):e109304. 2014-10-02 [PMID: 25275584] (IHC, Human)
• Gwendlyn Kollmorgen, Birgit Bossenmaier, Gerhard Niederfellner, Hans-Ulrich Häring, Reiner Lammers Structural Requirements for Cub Domain Containing Protein 1 (CDCP1) and Src Dependent Cell Transformation PLoS ONE 12/31/2012 [PMID: 23300860]
• Gwendlyn Kollmorgen, Birgit Bossenmaier, Gerhard Niederfellner, Hans-Ulrich Häring, Reiner Lammers Structural Requirements for Cub Domain Containing Protein 1 (CDCP1) and Src Dependent Cell Transformation PLoS ONE 2012-12-31 [PMID: 23300860] (Western Blot, Human)
• C H Benes, G Poulogiannis, L C Cantley, S P Soltoff The SRC-associated protein CUB Domain-Containing Protein-1 regulates adhesion and motility Oncogene 2/2/2012 [PMID: 21725358]
• C H Benes, G Poulogiannis, L C Cantley, S P Soltoff The SRC-associated protein CUB Domain-Containing Protein-1 regulates adhesion and motility Oncogene 2012-02-02 [PMID: 21725358] (Immunocytochemistry, Human)

Review for CDCP1 Antibody (MAB2666) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23300860
• Species: Human

Bioinformatics

• Gene Symbol: CDCP1
• Uniprot:
  • NP_073753()