FAF1 Antibody - BSA Free

Western Blot: FAF1 Antibody [NBP1-76745] - Analysis of FAF1 in THP-1 cell lysate with FAF1 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry: FAF1 Antibody - BSA Free [NBP1-76745] - Immunohistochemistry of FAF1 in rat spleen tissue with FAF1 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: FAF1 Antibody - BSA Free [NBP1-76745] - Immunofluorescence of FAF1 in rat spleen tissue with FAF1 antibody at 2 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FAF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FAF1 Antibody - BSA Free (NBP1-76745) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAF1. The immunogen is located within the last 50 amino acids of FAF1. Amino Acid Squence: STFPRRDVTQLDPNKSLLE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAF1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  FAF1 Peptide (NBP1-76745PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FAF1 Antibody - BSA Free

• CGI-03
• Fas (TNFRSF6) associated factor 1
• HFAF1s
• TNFRSF6-associated factor 1
• UBX domain protein 3A
• UBX domain-containing protein 12
• UBX domain-containing protein 3A
• UBXD12FLJ37524
• UBXN3AhFAF1FAS-associated factor 1

Background

Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain. Overexpression of FAF1 also decreases the basal level of NF-kappaB activity in transfected 293 cells, inhibits NF-kappaB activity induced by TNF-alpha, IL-1beta and lipopolysaccharide, and prevents NF-kappaB translocation to the nucleus, suggesting that another role of FAF1 is to negatively regulate the activity of NF-kappaB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway. Multiple differentially spliced isoforms of FAF1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAF1
• Uniprot:
  • NP_008982(Human)