Factor XII heavy chain Antibody (4E7) - BSA Free

Immunohistochemistry: Factor XII heavy chain Antibody (4E7) - BSA Free [NBP3-46534] - Immunohistochemistry of paraffin-embedded human liver tissue slide using (Factor XII heavy chain Antibody) at dilution of 1:200

Western Blot: Factor XII heavy chain Antibody (4E7) - BSA Free [NBP3-46534] - K-562 cells were subjected to SDS PAGE followed by western blot with (Factor XII heavy chain antibody) at dilution of 1:1000

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Factor XII heavy chain Antibody (4E7) - BSA Free Summary

• Immunogen: coagulation factor XII(Hageman factor). (Uniprot# P00748)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: F12
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:50-1:500
  • Western Blot 1:500-1:2000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Factor XII heavy chain Antibody (4E7) - BSA Free

• beta-factor XIIa part 1
• coagulation factor XII (Hageman factor)
• coagulation factor XII
• coagulation factor XIIa heavy chain
• coagulation factor XIIa light chain
• EC 3.4.21
• EC 3.4.21.38
• HAE3
• HAEX
• HAFbeta-factor XIIa part 2
• Hageman factor

Background

The F12 gene encodes a 615 amino acid long, 67 kDA serum glycoprotein coagulation factor XII that is eventually cleaved into four chains. Factor XII functions in the initiation of blood coagulation, fibrinolysis, as well as in the creation of bradykinin and angiotension. F12 participates in cell adhesion plasmin signaling, blood coagulation signaling pathways, MSP-RON signaling, intrinsic prothrombin activation pathways, and blood clotting cascades. It interacts with Genes CD93, MMP13, EPAS1, APP, and KLKB1. Defects in F12 lead to factor Xii deficiency (Hageman factor deficiency) as well as hereditary angioedema type 3 (estrogen-related HAE). F12 is also associated with blood clots, acute myocardial infarction, thrombosis, spastic paraplegia, angiodema, retinal vein occlusion, arterial calcification, heart disease, C1 inhibitor deficiency, hemorrhagic disease, and acalculous cholecystitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F12