Factor XII heavy chain Antibody (10-11-5) - BSA Free

Sandwich ELISA: Factor XII heavy chain Antibody (10-11-5) - BSA Free [NBP2-41375] - Analysis using the Biotin conjugate of NBP2-41375. The calibration curveof a sandwich assay using NBP2-41376B as the biotinylated detection antibody and NBP2-41377 as the capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 10-11-5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Factor XII heavy chain Antibody (10-11-5) - BSA Free Summary

• Immunogen: Affinity purified human factor XII.
• Specificity: Antibody binds the heavy chain of human factor XII, including factor XII derivatives such as a-factor XIIa and a-factor XIIa C1-inhibitor complexes.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: F12
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-41375 was used in ELISA. Biotinylated NBP2-41375 (1 ug/mL) was used as a detection antibody when used in combination with NBP2-41377 as a capture antibody (1.6 ug/mL) to measure Total alpha-factor XIIa and alpha-factor XIIa C1-inhibitor complexes). (1) WB: NBP2-41375 was used in western blot to detect factor XII, alpha-factor XIIa C1-inh complexes and alpha-factor XIIa.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Factor XII heavy chain Antibody (10-11-5) - BSA Free

• beta-factor XIIa part 1
• coagulation factor XII (Hageman factor)
• coagulation factor XII
• coagulation factor XIIa heavy chain
• coagulation factor XIIa light chain
• EC 3.4.21
• EC 3.4.21.38
• HAE3
• HAEX
• HAFbeta-factor XIIa part 2
• Hageman factor

Background

The F12 gene encodes a 615 amino acid long, 67 kDA serum glycoprotein coagulation factor XII that is eventually cleaved into four chains. Factor XII functions in the initiation of blood coagulation, fibrinolysis, as well as in the creation of bradykinin and angiotension. F12 participates in cell adhesion plasmin signaling, blood coagulation signaling pathways, MSP-RON signaling, intrinsic prothrombin activation pathways, and blood clotting cascades. It interacts with Genes CD93, MMP13, EPAS1, APP, and KLKB1. Defects in F12 lead to factor Xii deficiency (Hageman factor deficiency) as well as hereditary angioedema type 3 (estrogen-related HAE). F12 is also associated with blood clots, acute myocardial infarction, thrombosis, spastic paraplegia, angiodema, retinal vein occlusion, arterial calcification, heart disease, C1 inhibitor deficiency, hemorrhagic disease, and acalculous cholecystitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F12