FACA/FANCA Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

FACA/FANCA Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human FACA/FANCA
  Tyr35-Arg142
  Accession # O15360
• Specificity: Detects human FACA/FANCA in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse FANCA, recombinant human (rh) FANCD2, rhFANCE, rhFANCF, rhFANCG, and rhFANCI is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FACA/FANCA Antibody [Alexa Fluor® 594]

• FA
• FA1
• FAA
• FAAMGC75158
• FACA
• FACAFanconi anemia group A protein
• FAH
• FA-H
• FANCA
• FANCH
• Fanconi anemia, complementation group A
• Fanconi anemia, complementation group H
• Fanconi anemia, type 1
• Protein FACA

Background

FACA (Fanconi anemia group A) is a 160‑165 kDa protein that belongs to Fanconi anemia protein group I. FACA is widely expressed, activated by phosphorylation, partners with FANCG, and participates in the formation of an eight subunit Fanconi anemia core complex that ubiquitinates FANCD2 and FANCI. Ultimately, this contributes to the reactivation of stalled DNA repair replication forks. Human FACA is 1455 amino acids (aa) in length. It contains one NLS (aa 18‑34) and four phosphorylation sites at Ser849, 850, 858 and 1449. There are four potential splice variants. One shows a premature truncation after Cys297, a second contains a six aa substitution for aa 297‑1455, a third shows a deletion of aa 143‑174 accompanied by truncation after Cys297, and a fourth exhibits an alternative start site at Met528, accompanied by a 35 aa substitution for aa 1390‑1455. Over aa 35‑142, human FACA shares 61% aa identity with mouse FACA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.