Mouse FABP2/I-FABP ELISA Kit (Colorimetric)

ELISA: Mouse FABP2/I-FABP ELISA Kit (Colorimetric) [NBP2-82214] - Samples were spiked with high concentrations of Mouse FABP2/I-FABP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse FABP2/I-FABP ELISA Kit (Colorimetric) [NBP2-82214] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Mouse FABP2/I-FABP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse FABP2/I-FABP in samples. No significant cross-reactivity or interference between Mouse FABP2/I-FABP and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6%
• Intra-Assay: CV% < 6%
• Spike Recovery: average = 99% for serum; average = 96% for EDTA plasma; average = 94% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FABP2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse FABP2/I-FABP ELISA Kit (Colorimetric)

• FABP2
• FABPI
• fatty acid binding protein 2, intestinal
• Fatty acid-binding protein 2
• fatty acid-binding protein, intestinal
• IFABP
• I-FABP
• I-FABPMGC133132
• Intestinal FABP
• Intestinal-type fatty acid-binding protein

Background

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for FABP2/I-FABP ELISA Kit (NBP2-82214)(3)

Publications using NBP2-82214

• Samuelson D, Daniel Villageliu1 , Cunningham K et al. Regulation of Natural Killer Cell TGF-? and AhR Signaling Pathways Via the Intestinal Microbiota is Critical for Host Defense Against Alcohol-Associated Bacterial Pneumonia Research square 2023-10-18 [PMID: 37886455] (ELISA)
• Brandt, A;Kromm, F;Hernández-Arriaga, A;Martínez Sánchez, I;Bozkir, HÖ;Staltner, R;Baumann, A;Camarinha-Silva, A;Heijtz, RD;Bergheim, I; Cognitive Alterations in Old Mice Are Associated with Intestinal Barrier Dysfunction and Induced Toll-like Receptor 2 and 4 Signaling in Different Brain Regions Cells 2023-08-27 [PMID: 37681885] (ELISA)
• Li Y, Li H, Tong H et al. Outer membrane vesicles derived from heatstroke-associated intestinal microbiota promote multiple organ injury in mice Microbial pathogenesis 2022-06-23 [PMID: 35753600] (ELISA)

FAQs for FABP2/I-FABP ELISA Kit (NBP2-82214). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FABP2