FABP2/I-FABP Antibody (323730) [Unconjugated]

Western blot shows lysates of human small intestine tissue and mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FABP2/I-FABP Monoclonal Antibody (Catalog # MAB30781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP2/I-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FABP2/I‑FABP was detected in perfusion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human/Mouse FABP2/I‑FABP Monoclonal Antibody (Catalog # MAB30781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu
• Applications: WB, IHC, ELISA(Cap)
• Clone: 323730
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FABP2/I-FABP Antibody (323730) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FABP2/I‑FABP
  Ala2-Asp132
  Accession # P12104
• Specificity: Detects human FABP2/I‑FABP in ELISAs. Detects human and mouse FABP2/I‑FABP in Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human FABP1, 3, 5, 6, 7, 8, 9, recombinant mouse FABP4, 9, or recombinant rat FABP2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FABP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: ELISA Detection: Human FABP2/I-FABP Biotinylated Antibody (Catalog number BAF3078)
  Standard: Recombinant Human FABP2 (Catalog number 2694-CL/CF)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP2/I-FABP Antibody (323730) [Unconjugated]

• FABP2
• FABPI
• fatty acid binding protein 2, intestinal
• Fatty acid-binding protein 2
• fatty acid-binding protein, intestinal
• IFABP
• I-FABP
• I-FABPMGC133132
• Intestinal FABP
• Intestinal-type fatty acid-binding protein

Background

Fatty acid binding protein-2 (FABP2; also I- or intestinal FABP) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1‑3). FABP2 is one of nine cytoplasmic FABPs that are 14‑15 kDa in size and range from 126‑134 amino acids (aa) in length (2). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Nevertheless, based on aa sequence, the nine FABP family members have been shown to form three subgroups, with FABP2/I-FABP linked with liver/L-FABP and heart/H-FABP (2). The designation of a tissue type, such as intestinal, does not suggest the binding protein is universally expressed in all cell types that make up the organ or tissue. Human I-FABP, the product of the FABP-2 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure (called a beta -clam) generated by a series of antiparallel beta -strands and two alpha -helices (1, 2, 4). Preferred ligands for FABP2 include sixteen to twenty carbon long chain fatty acids (4). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in “internalization” of the ligand.(1) An Ala-to-Thr polymorphism at position # 54 has been reported to potentially impact FABP2 function (2). This polymorphism has been suggested to be associated with an increased risk of type II diabetes. To date, the evidence appears to be equivocal (1, 2). This polymorphism may, however, have unusual metabolic effects depending upon the type of diet involved (1, 5). Human FABP-2 is 78%, 82% and 86% aa identical to mouse, rat and canine FABP2, respectively. It also shows 33% and 24% aa identity to human H-FABP and L‑FABP, respectively. FABP2 is proposed to transport fatty acids (FA) into cells, increase FA availability to enzymes, protect cell structures from FA attack, and target FA to transcription factors in the nuclear lumen (3).

1. Weiss, E.P. et al. (2002) Physiol. Genomics 10:145.
2. Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
3. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
4. Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
5. Dworatzek, P. et al. (2004) Am. J. Clin. Nutr. 79:1110.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FABP2
• Uniprot:
  • P12104()