Recombinant Mouse FABP1/L-FABP Protein

SDS-Page: Mouse FABP1/L-FABP Protein [NBP2-35180]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse FABP1/L-FABP Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 127 amino acids corresponding to FABP1/L-FABP Source: E. coli

  Uniprot ID: P12710

  Amino Acid Sequence: MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI

• Details of Functionality: liver FABP Protein is fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 uM rMuFABP1 is achieved with approximately 5 uM cis-paranaric acid.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FABP1
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of FABP1/L-FABP as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 14.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 2 % trehalose.
• Preservative: Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse FABP1/L-FABP Protein

• FABP1
• FABPL
• fatty acid binding protein 1, liver
• fatty acid-binding protein, liver
• LFABP
• L-FABP
• L-FABPFatty acid-binding protein 1
• Liver-type fatty acid-binding protein

Background

The fatty-acid-binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids. These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular membranes. FABP (FABP1) encoded by the FABP1 gene, also known as liver-type fatty acid-binding protein (L-FABP), is a member of FABP family and it is a small, highly conserved, cytoplasmic proteins. In addition, FABP (FABP1) binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. Furthermore, it may be involved in intracellular lipid transport. Through amino acid sequence comparison, murine FABP (FABP1) shares 84 % and 94 % a.a. sequence identity with human and rat FABP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FABP1
• Uniprot:
  • P12710(Human)