FABP1/L-FABP Antibody (965616) [Unconjugated]

FABP1/L‑FABP was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to hepatocytes using Mouse Anti-Human FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29644) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human colon tissue, and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29644) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 965616
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FABP1/L-FABP Antibody (965616) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FABP1/L-FABP
  Met1-Ile127
  Accession # P07148
• Specificity: Detects human FABP-1/L-FABP in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Immunocytochemistry 2-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP1/L-FABP Antibody (965616) [Unconjugated]

• FABP1
• FABPL
• fatty acid binding protein 1, liver
• fatty acid-binding protein, liver
• LFABP
• L-FABP
• L-FABPFatty acid-binding protein 1
• Liver-type fatty acid-binding protein

Background

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P07148()