F4/80 Antibody (CI-A3-1)

Images

 
Immunocytochemistry/ Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Lymph node stained for macrophages, red and B cells CD79b, green; nuclei are stained with DAPI, blue.
Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Mouse spleen stained with A: Rat anti Mouse. B: CI-A3-1 clone preincubated with 2 molar excess of human anti Idiotypic.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - A surface stain was performed on RAW 246.7 cells with F4/80 antibody (CI-A3-1) NB600-404AF488 (blue) and a matched isotype control (orange). Cells were incubated in ...read more
Immunocytochemistry/ Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse small intestine.
Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Frozen mouse spleen with F4/80 anti-mouse antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with rat anti-mouse F4/80 antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with F4/80 antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with F4/80 antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse J774 cells with F4/80 antibody clone C1-A3-1.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with F4/80 antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages cells with rat anti-mouse F4/80 antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages cells with rat anti-mouse F4/80 antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with F4/80 antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with clone CI-A3-1 F4/80 antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
Clone
CI-A3-1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

F4/80 Antibody (CI-A3-1) Summary

Immunogen
Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Localization
Cell surface
Marker
Macrophage Marker
Specificity
This recognizes mouse F4/80, a 160kD glycoprotein expressed by murine macrophages.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
EMR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Electron Microscopy 1:10-1:500
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Radioimmunoassay 1:100-1:2000
Application Notes
This product requires pretreatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours.
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB600-404 in the following applications:

Publications
Read Publications using
NB600-404 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26512116).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for F4/80 Antibody (CI-A3-1)

  • cell surface glycoprotein F4/80
  • DD7A5-7
  • EGF-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EGF-TM7
  • EMR1 hormone receptor
  • F4/80
  • Gpf480
  • Ly71
  • lymphocyte antigen 71
  • Mouse EMR1
  • Rat EMR1
  • TM7LN3

Background

F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGFTM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for F4/80 Antibody (NB600-404)(26)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(4)
IHC-Fr
(4)
IHC-P
(7)
WB
(1)
All Applications
Filter By Species
Mouse
(14)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB600-404 Applications Species
Moon H, Donahue LR, Choi E et al. Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure. Cell Stem Cell. 2017 Oct 04 [PMID: 29033353] (WB) WB
Potula HH, Richer L, Werts C, Gomes-Solecki M. Pre-treatment with Lactobacillus plantarum prevents severe pathogenesis in mice infected with Leptospira interrogans and may be associated with recruitment of myeloid cells PLoS Negl Trop Dis 2017 Aug 25 [PMID: 28841659] (IHC-P, Mouse) IHC-P Mouse
Yuan H, Jiang W, von Roemeling CA et al. Multivalent bi-specific nanobioconjugate engager for targeted cancer immunotherapy. Nat Nanotechnol May 1 2017 [PMID: 28459470] (IHC-P) IHC-P
Chung KJ, Chatzigeorgiou A, Economopoulou M et al. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity. Nat. Immunol. Apr 17 2017 [PMID: 28414311]
Wang W, Xu MJ, Cai Y et al. Inflammation is independent of steatosis in a murine model of steatohepatitis.. Hepatology. Feb 21 2017 [PMID: 28220523] (IHC-P, Mouse) IHC-P Mouse
Demonbreun AR, Allen MV, Warner JL et al. Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal Disruption. Am. J. Pathol. 2016 Apr 09 [PMID: 27070822] (IHC-Fr, ICC/IF, Mouse)

Details:
This publication used the AF488 conjugated form of this antibody (NB600-404AF488)
IHC-Fr, ICC/IF Mouse
Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015 Sep 24 [PMID: 26400209] (IHC, Mouse) IHC Mouse
Merches K, Khairnar V, Knuschke T et al. Virus-Induced Type I Interferon Deteriorates Control of Systemic Pseudomonas Aeruginosa Infection Cell Physiol Biochem 2015 Aug 17 [PMID: 26279441] (IHC-Fr, Mouse)

Details:
This citation used the DyLight 488 version of this antibody.
IHC-Fr Mouse
Saleh MA, McMaster WG, Wu J et al. Lymphocyte adaptor protein LNK deficiency exacerbates hypertension and end-organ inflammation J. Clin. Invest. 2015 Mar 02 [PMID: 25664851] (IHC-P, Mouse) IHC-P Mouse
Nishiwaki S, Nakayama T, Murata M et al. Dexamethasone Palmitate Ameliorates Macrophages-Rich Graft-versus-Host Disease by Inhibiting Macrophage Functions. PLoS ONE. 2014 May 08 [PMID: 24806147] (IHC-P, Mouse) IHC-P Mouse
Show All 26 Publications.

Review for F4/80 Antibody (NB600-404) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB600-404:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin F4/80 NB600-404
Enlarge
reviewed by:
Naiche Adler
IHC-P Mouse 03/26/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse lymph node FFPE
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for F4/80 Antibody (NB600-404). (Showing 1 - 4 of 4 FAQs).

  1. Are the F4/80/EMR1 antibodies validated in Simple Western?
    • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
  2. Does F4/80/EMR1 antibodies come in lyophilized form?
    • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
  3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
    • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
  4. I'm hoping to use F4/80 as a macrophage marker in skeletal muscle using IHC-Fr but I haven't seen this done successfully (and heard people have tried without much luck). Do you know of any validation that has been done on that tissue?
    • We have not validated NB600-404 on skeletal muscle specifically, and we haven't yet had any customer feedback regarding this antibody being used on skeletal muscle. If you are interested in using this antibody for your testing, and have any trouble getting it to work for you, please let us know. We do offer a guarantee on our products for the species and applications listed, and if we can't get this product working for you, we will compensate you for the purchase.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-404AF405
Alexa Fluor 488 Labeled NB600-404AF488
Alexa Fluor 647 Labeled NB600-404AF647
Alexa Fluor 700 Labeled NB600-404AF700
Allophycocyanin Labeled NB600-404APC
DyLight 350 Labeled NB600-404UV
DyLight 405 Labeled NB600-404V
DyLight 488 Labeled NB600-404G
DyLight 680 Labeled NB600-404FR
DyLight 755 Labeled NB600-404IR
PE Labeled NB600-404PE
PerCP Labeled NB600-404PCP

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F4/80 NB600-404

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Recent Reviews

4
5
0
4
1
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0
2
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1
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Naiche Adler
03/26/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol EMR1
Entrez
Uniprot