F4/80 Antibody (CI-A3-1) - BSA Free

Images

 
Immunohistochemistry: F4/80 Antibody (CI-A3-1) [NB600-404] - Oral treatment with L plantarum leads to recruitment of myeloid cells in kidney.Immunostaining of kidney sections from groups of treated mice, in the presence ...read more
Immunocytochemistry/ Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Lymph node stained for macrophages, red and B cells CD79b, green; nuclei are stained with DAPI, blue.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - A surface stain was performed on RAW 246.7 cells with F4/80 antibody (CI-A3-1) NB600-404AF488 (blue) and a matched isotype control (orange). Cells were incubated in ...read more
Immunohistochemistry-Paraffin: F4/80 Antibody (CI-A3-1) [NB600-404] - pNAPE-EcN reduces diet-induced hepatic inflammation. Representative images of liver sections immunostained for F4/80. Image collected and cropped by ...read more
Immunocytochemistry/ Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Analysis of monocytes cultured in M-CSF using F4/80 antibody. Primary antibody dilution: 1:200. Overnight incubation in RT. Image from ...read more
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404]
Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Frozen mouse spleen with F4/80 anti-mouse antibody.
Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Mouse spleen stained with A: Rat anti Mouse. B: CI-A3-1 clone preincubated with 2 molar excess of human anti Idiotypic.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with F4/80 antibody.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with clone CI-A3-1 F4/80 antibody.

Product Details

Summary
Reactivity Hu, Mu, Ce, EcSpecies Glossary
Applications WB, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
Clone
CI-A3-1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

F4/80 Antibody (CI-A3-1) - BSA Free Summary

Immunogen
This F4/80 Antibody (CI-A3-1) was developed against Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Localization
Cell surface
Marker
Macrophage Marker
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
Adgre1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy 1:10-1:500
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000
  • Radioimmunoassay 1:100-1:2000
  • Western Blot 1:100-1:2000
Application Notes
This product requires pretreatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. E. coli reactivity reported in scientific literature (PMID:31181736).
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NB600-404 in the following applications:

Publications
Read Publications using
NB600-404 in the following applications:

  • 1 publication
  • 1 publication
  • ICC
    1 publication
  • 4 publications
  • IHC
    17 publications
  • 5 publications
  • 22 publications
  • SW
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for F4/80 Antibody (CI-A3-1) - BSA Free

  • ADGRE1
  • cell surface glycoprotein F4/80
  • DD7A5-
  • DD7A5-7
  • EGF-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EGF-TM7
  • Emr
  • EMR1 hormone receptor
  • Emr1
  • F4/80
  • Gpf480
  • Ly7
  • Ly71
  • lymphocyte antigen 71
  • Mouse EMR1
  • Rat EMR1
  • TM7L
  • TM7LN3

Background

F4/80 antigen is a cell surface glycoprotein that is the murine homolog of human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) (1,2). Specifically, F4/80 (EMR1) is one of five members belonging to the adhesion G-coupled receptor (ADGRE) subfamily, also known as the epidermal growth factor seven transmembrane (EGF-TM7) receptors (1,2). F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).

Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).

References

1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7

2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA

Publications for F4/80 Antibody (NB600-404)(73)

We have publications tested in 4 confirmed species: Human, Mouse, C. elegans, E. coli.

We have publications tested in 9 applications: FLOW, Flow, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, SW, WB.


Filter By Application
FLOW
(1)
Flow
(1)
ICC
(1)
ICC/IF
(4)
IHC
(17)
IHC-Fr
(5)
IHC-P
(22)
SW
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(46)
C. elegans
(1)
E. coli
(1)
All Species
Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using NB600-404 Applications Species
Chen L, Wu H, Ren C, et al. Inhibition of PDGF-BB reduces alkali-induced corneal neovascularization in mice Molecular Medicine Reports Jan 28 2021 [PMID: 33537811]
Su W, Sun S, Tian B et al. Efficacious, safe and stable inhibition of corneal neovascularization by AAV vectored anti-VEGF therapeutics Molecular Therapy - Methods & Clinical Development Jun 1 2021
Dai Q, Zhang Y, Liao X et al. Fluorofenidone Alleviates Renal Fibrosis by Inhibiting Necroptosis Through RIPK3/MLKL Pathway Frontiers in pharmacology Dec 16 2020 [PMID: 33390935] (IHC-P, Mouse) IHC-P Mouse
Nishiwaki S, Saito S, Takeshita K et al. In vivo tracking of transplanted macrophages with near infrared fluorescent dye reveals temporal distribution and specific homing in the liver that can be perturbed by clodronate liposomes PloS one Dec 10 2020 [PMID: 33301448] (IHC-P, Mouse) IHC-P Mouse
Wang L, Sinnott-Armstrong N, Wagschal A et al. A MicroRNA Linking Human Positive Selection and Metabolic Disorders Cell Oct 29 2020 [PMID: 33058756] (Mouse) Mouse
Li Z, Bian X, Ma Y et al. Uterine scar leads to adverse pregnant consequence through impairing the response of endometrium to steroids Endocrinology Sep 25 2020 [PMID: 32976565] (Human) Human
Rai V, Wood MB, Feng H et al. The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells Scientific reports Sep 16 2020 [PMID: 32938973] (IHC-Fr, Mouse) IHC-Fr Mouse
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (Flow, Mouse)

Details:
Citation using the Allophycocyanin format of this antibody.
Flow Mouse
Hozain S, Cottrell J CDllb+ targeted depletion of macrophages negatively affects bone fracture healing Bone Jun 12 2020 [PMID: 32535290] (IHC-P, Mouse) IHC-P Mouse
Mokshagundam S, Ding T, Rumph JT et al. Developmental 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure of either parent enhances the risk of necrotizing enterocolitis in neonatal mice Birth Defects Res Jun 9 2020 [PMID: 32519502]
Show All 73 Publications.

Reviews for F4/80 Antibody (NB600-404) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB600-404:
Filter by Applications
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry F4/80 NB600-404
Enlarge
4
reviewed by:
Mozhdeh Sojoodi
ICC Mouse 02/07/2022
View

Summary

ApplicationImmunocytochemistry
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
Lot1806

Comments

Comments1:200 Dilution
Overnight incubation in RT
Immunohistochemistry-Paraffin F4/80 NB600-404
Enlarge
4
reviewed by:
Naiche Adler
IHC-P Mouse 03/26/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse lymph node FFPE
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for F4/80 Antibody (NB600-404). (Showing 1 - 4 of 4 FAQs).

  1. Are the F4/80/EMR1 antibodies validated in Simple Western?
    • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
  2. Does F4/80/EMR1 antibodies come in lyophilized form?
    • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
  3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
    • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
  4. I'm hoping to use F4/80 as a macrophage marker in skeletal muscle using IHC-Fr but I haven't seen this done successfully (and heard people have tried without much luck). Do you know of any validation that has been done on that tissue?
    • We have not validated NB600-404 on skeletal muscle specifically, and we haven't yet had any customer feedback regarding this antibody being used on skeletal muscle. If you are interested in using this antibody for your testing, and have any trouble getting it to work for you, please let us know. We do offer a guarantee on our products for the species and applications listed, and if we can't get this product working for you, we will compensate you for the purchase.

Secondary Antibodies

 

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Mozhdeh Sojoodi
02/07/2022
Application: ICC
Species: Mouse

Naiche Adler
03/26/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol Adgre1
Entrez
Uniprot