F4/80 Antibody (CI-A3-1)


Immunocytochemistry/ Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Lymph node stained for macrophages, red and B cells CD79b, green; nuclei are stained with DAPI, blue
Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Mouse spleen stained with A: Rat anti Mouse. B: Rat anti Mouse preincubated with 2 molar excess of Human anti Idiotypic.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with Rat anti Mouse.
Immunocytochemistry/ Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse small intestine.
Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Frozen mouse spleen with Rat anti Mouse.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with Rat anti Mouse.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with Rat anti Mouse.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with Rat anti Mouse.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse J774 cells with Rat anti Mouse.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with Rat anti Mouse.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages cells with Rat anti Mouse.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages cells with Rat anti Mouse.
Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with Rat anti Mouse.

Product Details

Reactivity MuSpecies Glossary
Applications WB, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
1.0 mg/ml

F4/80 Antibody (CI-A3-1) Summary

Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Cell surface
Macrophage Marker
This recognizes mouse F4/80, a 160kD glycoprotein expressed by murine macrophages.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS [pH7.4]
0.09% Sodium Azide
1.0 mg/ml
Protein G purified


Application Notes
This product requires pretreatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
160 kDa
Reviewed Applications
Read 1 Review rated 4
NB600-404 in the following application:

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NB600-404 in the following applications:

Alternate Names for F4/80 Antibody (CI-A3-1)

  • cell surface glycoprotein F4/80
  • DD7A5-7
  • EGF-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EGF-TM7
  • EMR1 hormone receptor
  • F4/80
  • Gpf480
  • Ly71
  • lymphocyte antigen 71
  • Mouse EMR1
  • Rat EMR1
  • TM7LN3


F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGFTM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for F4/80 Antibody (NB600-404)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P.

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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB600-404 Applications Species
Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015 Sep 24 [PMID:26400209] (IHC, Mouse) IHC Mouse
Saleh MA, McMaster WG, Wu J et al. Lymphocyte adaptor protein LNK deficiency exacerbates hypertension and end-organ inflammation J. Clin. Invest. 2015 Mar 02 [PMID:25664851] (IHC-P, Mouse) IHC-P Mouse
Nishiwaki S, Nakayama T, Murata M et al. Dexamethasone Palmitate Ameliorates Macrophages-Rich Graft-versus-Host Disease by Inhibiting Macrophage Functions. PLoS ONE. 2014 May 08 [PMID:24806147] (IHC-P, Mouse) IHC-P Mouse
Kwon HJ, Won YS, Park O et al. Aldehyde dehydrogenase 2 deficiency ameliorates alcoholic fatty liver but worsens liver inflammation and fibrosis in mice. Hepatology 2014 Feb 4 [PMID:24492981] (IHC-P, Mouse) IHC-P Mouse
Oh JY, Lee HJ, Ko AY et al. Analysis of macrophage phenotype in rejected corneal allografts. Invest Ophthalmol Vis Sci. 2013 Oct 24 [PMID:24159090]
Phieler J, Chung KJ, Chatzigeorgiou A et al. The complement anaphylatoxin c5a receptor contributes to obese adipose tissue inflammation and insulin resistance. J Immunol. 2013 Oct 15 [PMID:24043887] (IHC-P, Mouse) IHC-P Mouse
Crowder SW, Horton LW, Lee SH et al. Passage-dependent cancerous transformation of human mesenchymal stem cells under carcinogenic hypoxia FASEB J 2013 Apr 15 [PMID:23568779] (IHC-P, Human) IHC-P Human
Xiao C, Wang RH, Lahusen TJ et al. Progression of Chronic Liver Inflammation and Fibrosis Driven by Activation of c-JUN Signaling in Sirt6 Mutant Mice J Biol Chem 2012 Oct 16 [PMID:23076146] (IHC, Mouse) IHC Mouse
Karasawa F, Shiota A, Goso Y et al. Essential role of gastric gland mucin in preventing gastric cancer in mice J Clin Invest 2012 Mar 1 [PMID:22307328] (IHC, Mouse) IHC Mouse
Ouvrier A, Alves G, Damon-SoubeyrC et al. Dietary cholesterol-induced post-testicular infertility. PLoS ONE 20116(11):e26966. [PMID:22073227]
Show All 21 Publications.

Reviews for F4/80 Antibody (NB600-404) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-404:
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Images Ratings Applications Species Date Details
 F4/80 NB600-404
reviewed by:
Mouse 03/26/2015


Sample Testedmouse lymph node FFPE

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for F4/80 Antibody (NB600-404). (Showing 1 - 4 of 4 FAQs).

  1. Are the F4/80/EMR1 antibodies validated in Simple Western?
    • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
  2. Does F4/80/EMR1 antibodies come in lyophilized form?
    • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
  3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
    • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
  4. I'm hoping to use F4/80 as a macrophage marker in skeletal muscle using IHC-Fr but I haven't seen this done successfully (and heard people have tried without much luck). Do you know of any validation that has been done on that tissue?
    • We have not validated NB600-404 on skeletal muscle specifically, and we haven't yet had any customer feedback regarding this antibody being used on skeletal muscle. If you are interested in using this antibody for your testing, and have any trouble getting it to work for you, please let us know. We do offer a guarantee on our products for the species and applications listed, and if we can't get this product working for you, we will compensate you for the purchase.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-404AF405
Alexa Fluor (R) 488 Labeled NB600-404AF488
Alexa Fluor (R) 647 Labeled NB600-404AF647
Alexa Fluor (R) 700 Labeled NB600-404AF700
Allophycocyanin Labeled NB600-404APC
DyLight 350 Labeled NB600-404UV
DyLight 405 Labeled NB600-404V
DyLight 405LS Labeled NB600-404V3
DyLight 488 Labeled NB600-404G
DyLight 680 Labeled NB600-404FR
DyLight 755 Labeled NB600-404IR
PE Labeled NB600-404PE
PerCP Labeled NB600-404PCP

Additional F4/80 Antibody (CI-A3-1) Products

F4/80 NB600-404

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Contact Information

Product PDFs


Gene Symbol EMR1

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