EZH2/KMT6 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

EZH2/KMT6 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human EZH2
  Gly512-Ile645
  Accession # Q15910
• Specificity: Detects human and mouse EZH2 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EZH2/KMT6 Antibody [Alexa Fluor® 405]

• EC 2.1.1
• EC 2.1.1.43
• enhancer of zeste (Drosophila) homolog 2
• enhancer of zeste 2
• enhancer of zeste homolog 2 (Drosophila)
• Enhancer of zeste homolog 2
• ENX1
• ENX-1KMT6EZH1KMT6A
• EZH2
• histone-lysine N-methyltransferase EZH2
• KMT6
• Lysine N-methyltransferase 6
• MGC9169

Background

EZH2 (Enhancer of zeste homolog 2; also ENX-1 and Lys N-methyltransferase 6) is an 80 kDa member of the EZ family of chromatin-dependent gene regulators. It is a nuclear protein that represses gene transcription through histone methylation. Human EZH2 is 746 amino acids (aa) in length. It contains an NLS (aa 490‑495), a Cys-rich region, and a methyltransferase SET (Suppressor/Enhancer/Trithorax) domain (aa 606‑729). There are four potential splice variants. One shows a premature truncation after Cys286, a second shows a 6 aa substitution for aa 329‑746, a third shows a deletion of aa 83‑121, and the fourth exhibits a 5 aa insertion after His297. Over aa 512‑645, human, mouse and canine EZH2 are identical in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.