EYA1 Antibody

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Western Blot: EYA1 Antibody [NBP1-46136] - (2ug/ml) Analysis of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

EYA1 Antibody Summary

Immunogen
Peptide with sequence C-TDPTAEYSTIHSP corresponding to internal region according to NP_000494.2, NP_742056.1.
Specificity
This antibody is expected to recognize all isoforms (NP_742057.1; NP_000494.2; NP_742056.1). Reported variants represent identical protein: NP_000494.2, NP_742055.1
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EYA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 55 kDa band observed in lysates of cell lline HEK293 (calculated MW of 60.7 kDa band according to NP_742056.1).
Theoretical MW
64.593 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-46136.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EYA1 Antibody

  • BOP
  • BOR
  • EC 3.1.3.48
  • eyes absent (Drosophila) homolog 1
  • eyes absent homolog 1 (Drosophila)
  • eyes absent homolog 1
  • MGC141875

Background

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Four transcript variants encoding three distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EYA1 Antibody (NBP1-46136)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EYA1
Entrez
Uniprot