EYA1 Antibody

Western Blot: EYA1 Antibody [NBP1-46136] - (2ug/ml) Analysis of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EYA1 Antibody Summary

• Description: Novus Biologicals Goat EYA1 Antibody (NBP1-46136) is a polyclonal antibody validated for use in WB and ELISA. Anti-EYA1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TDPTAEYSTIHSP corresponding to internal region according to NP_000494.2, NP_742056.1.
• Specificity: This antibody is expected to recognize all isoforms (NP_742057.1; NP_000494.2; NP_742056.1). Reported variants represent identical protein: NP_000494.2, NP_742055.1
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EYA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in lysates of cell lline HEK293 (calculated MW of 60.7 kDa band according to NP_742056.1).
• Theoretical MW: 64.593 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EYA1 Antibody

• BOP
• BOR
• EC 3.1.3.48
• eyes absent (Drosophila) homolog 1
• eyes absent homolog 1 (Drosophila)
• eyes absent homolog 1
• MGC141875

Background

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Four transcript variants encoding three distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EYA1 Antibody (NBP1-46136)(1)

Publication using NBP1-46136

• Orten DJ, Fischer SM, Sorensen JL et al. Branchio-oto-renal syndrome (BOR): novel mutations in the EYA1 gene, and a review of the mutational genetics of BOR. Hum Mutat 2008-04-01 [PMID: 18220287]

Bioinformatics

• Gene Symbol: EYA1
• Entrez:
  • 2138(Human)
  • 14048(Mouse)
  • 502935(Rat)
• Uniprot:
  • Q99502(Human)