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ETV7 Antibody (OTI3B2)

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Western Blot: ETV7 Antibody (3B2) [NBP2-45544] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETV7.
Western Blot: ETV7 Antibody (3B2) [NBP2-45544] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETV7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
OTI3B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71938AF350
Alexa Fluor 405
NBP2-71938AF405
Alexa Fluor 488
NBP2-71938AF488
Alexa Fluor 532
NBP2-71938AF532
Alexa Fluor 594
NBP2-71938AF594
Alexa Fluor 647
NBP2-71938AF647
Alexa Fluor 700
NBP2-71938AF700
Alexa Fluor 750
NBP2-71938AF750
Biotin
NBP2-71938B
DyLight 350
NBP2-71938UV
DyLight 405
NBP2-71938V
DyLight 488
NBP2-71938G
DyLight 550
NBP2-71938R
DyLight 594
NBP2-71938DL594
DyLight 650
NBP2-71938C
DyLight 680
NBP2-71938FR
DyLight 755
NBP2-71938IR
HRP
NBP2-71938H
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71938
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ETV7 Antibody (OTI3B2) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 90-341 of human ETV7 (NP_057219) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ETV7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
Theoretical MW
38.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ETV7 Antibody (OTI3B2)

  • ETS translocation variant 7
  • ets variant 7
  • ets variant gene 7 (TEL2 oncogene)
  • TEL2 oncogene
  • TEL-2
  • TEL2ETS-related protein Tel2
  • TELBEts transcription factor TEL-2b
  • Tel-related Ets factor
  • transcription factor ets
  • transcription factor ETV7
  • Transcription factor Tel-2
  • TREF

Background

The ETS (E26 transformation-specific) family of transcription factors, of which ETV7 is a member, is a large group of evolutionarily conserved transcriptional regulators that play an important role in a variety of cellular processes throughout development and differentiation, and are involved in oncogenesis as well (Potter et al., 2000 (PubMed 10828014)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: ETV6/Tel Antibody [NBP1-80695] - Analysis in human cell lines U2OS and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695] - Staining of human breast shows high expression.
Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695] - Staining of human kidney shows low expression as expected.
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Flow Cytometry: ATM Antibody [NB100-104] - Analysis of PE conjugate of ATM Antibody [NB100-104PE]. An intracellular stain was performed on HeLa cells with ATM antibody [NB100-104PE] (blue) and a matched isotype control [NBP2-24893PE] (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.
Immunohistochemistry-Paraffin: ATM Antibody [NB100-104] - Staining of human tonsil, germinal center and mantle zone with ATM Antibody [NB100-104].
Flow Cytometry: ATM Antibody [NB100-104] - An intracellular stain was performed on HepG2 cells with PE-conjugated ATM antibody [NB100-104PE] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
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NB100-308
Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.
Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.
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Antibody (200C1339(SB ...
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Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Human colon stained with TEM8 antibody at 2.5 ug/ml concentration using DAB chromogen detection method (10X magnification)
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).
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NBP2-03417
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - Analysis of Jurkat cells, using anti-MMAB antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody, and then analyzed by flow cytometry.
Western Blot: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB.
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Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY licence.
N/A: TOR/mTOR [p Ser2448] Antibody [NB600-607]
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Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, mouse placenta tissue, mouse uterus tissue, and rat ovary tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TRRAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TRRAP at approximately 440 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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H00023049-M02
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(OTI1A6)
NBP2-01764
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Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2.
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TGF-beta RIII
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Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Detection of Human Sin1 on HeLa whole cell lysate.
Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NB110-40424] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- Sin1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
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(7 Publications)
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Applications: IHC, IHC-P, IP, WB
HSP90 alpha
Antibody - BSA Free
NBP1-77685
Simple Western: HSP90 alpha Antibody [NBP1-77685] - Image shows a specific band for Hsp90A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77685] - HSP901a antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: HSP90 alpha Antibody [NBP1-77685] - Staining of human kidney carcinoma in HSP901A using DAB with hematoxylin counterstain.
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ETV7 Antibody
(OTI3B2)
NBP2-45544
ETV7 Antibody (OTI3B2)
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol ETV7