ETFB Antibody - BSA Free

Immunoprecipitation:ETFB AntibodyNBP3-29871] -Detection of human ETFB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ETFB antibody NBP3-29871 (lot NBP3-29871-1) used for IP at 6 µg per reaction. ETFB was also immunoprecipitated by rabbit anti-ETFB.. For blotting immunoprecipitated ETFB, NBP3-29871 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ETFB Antibody - BSA Free Summary

• Immunogen: Between 205 and 255
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ETFB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for ETFB Antibody - BSA Free

• beta-ETF
• electron transfer flavoprotein beta subunit
• electron transfer flavoprotein beta-subunit
• electron transfer flavoprotein subunit beta
• electron transfer flavoprotein, beta polypeptide
• electron-transfer-flavoprotein, beta polypeptide
• electron-transferring-flavoprotein, beta polypeptide
• MADD

Background

ETFB is a is an electron-transfer-flavoprotein, (beta polypeptide), and a subcomponent of Electron transfer flavoprotein (ETF). ETF exists in the mitochondrial matrix as a heterodimer of 30-kD alpha subunits (ETFA) and 28-kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. This electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases and transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ETFB