ETFA Antibody

Images

 
Western Blot: ETFA Antibody [NBP1-84857] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84857] - Staining of human kidney.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84857] - Staining of human duodenum shows strong cytoplasmic positivity in granular pattern in glandular cells.
Independent Antibodies: Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84857] - Staining of human colon, kidney, liver and pancreas using Anti-ETFA antibody NBP1-84857 (A) shows similar protein distribution ...read more
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84857] - Staining of human pancreas.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84857] - Staining of human colon.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84857] - Staining of human liver.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

ETFA Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAAT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETFA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
ETFA Protein (NBP1-84857PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ETFA Antibody

  • alpha-ETF
  • electron transfer flavoprotein alpha-subunit
  • Electron transfer flavoprotein subunit alpha, mitochondrial
  • electron-transfer-flavoprotein, alpha polypeptide
  • EMA
  • GA2
  • glutaric aciduria II
  • MADD

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ETFA