Erythropoietin R Antibody

Product Discontinued

Erythropoietin R Antibody Summary

• Immunogen: The immunogen used was the cDNA of the extracellular domain of human EpoR expressed as a protein in E. coli.
• Specificity: Antibody titers and specificity were analyzed by ELISA assay using control samples by varying concentration of EpoR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: EPOR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry Affinity purified antibody was found to detect EpoR at concentrations of 50 ng/ml. Neutralization of ligand binding has been detected using erythroleukemia cell line TF-1 with 3000 Epo receptors/cell to block Iodine 125 labeled erythropoietin ligand binding. This antibody will recognize EpoR at its migration of 49 kD on SDS-PAGE. High quality secondary anti-sheep reagents are essential for this assay. Past experiments recommend the use of rabbit anti-sheep rather than donkey anti-sheep antibodies as a secondary labeling reagents.

Reactivity Notes

Reacts with human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.15M PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 1.32 mg/ml
• Purity: Protein G purified

Alternate Names for Erythropoietin R Antibody

• EpoR
• EPO-R
• Erythropoietin R
• erythropoietin receptor
• MGC138358

Background

The human erythropoietin receptor (EpoR) is located on stem and mature cells of the hematopoietic system. When activated by its native ligand, erythropoietin (Epo), EpoR mediates proliferation and maturation of the erythroid precursor cells leading to expansion of red cell populations. Human recombinant Epo is currently used for treatment of patients undergoing erythrocyte depletion due to dialysis for kidney failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Erythropoietin R Antibody (NB120-26824)(3)

Publications using NB120-26824

• Nakatsuji T. Long-term survival of a piece of rat bone marrow graft with the bone in syngeneic rat spleen: new roles of bone marrow mast cells host spleen megakaryocytes secreting vascular endothelial growth factor (VEGF). Haema; 8(3):450-457. 2005-01-01
• Fairchild Benyo D, Conrad KP. Expression of the erythropoietin receptor by trophoblast cellsin the human placenta. Biol Reprod;60(4):861-70. 1999-04-01 [PMID: 10084959]
• Harris KW, Mitchell RA, Winkelmann JC. Lig binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli. J Biol Chem;267(21):15205-9. 1992-07-25 [PMID: 1321832]

Bioinformatics

• Gene Symbol: EPOR
• Entrez:
  • 2057(Human)