Erythropoietin R Antibody (713210) [Unconjugated]

Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) induces proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin (Tissue Culture Grade) (0.278 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3073). The ND50 is typically0.4-2.4 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 713210
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Erythropoietin R Antibody (713210) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
  Ala25-Pro250
  Accession # P19235
• Specificity: Detects human Erythropoietin R in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.4-2.4 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin R Antibody (713210) [Unconjugated]

• EpoR
• EPO-R
• Erythropoietin R
• erythropoietin receptor
• MGC138358

Background

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P19235()