Erythropoietin R Antibody (690748) [Unconjugated]

Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for Erythropoietin R at approximately 64 to 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

KU812 cells were stained with Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3072, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: 690748
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Erythropoietin R Antibody (690748) [Unconjugated] Summary

• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Y426 of human Erythropoietin R
  Accession # P19235
• Specificity: Detects human Erythropoietin R in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPOR
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin R Antibody (690748) [Unconjugated]

• EpoR
• EPO-R
• Erythropoietin R
• erythropoietin receptor
• MGC138358

Background

Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the Erythropoietin Receptor (Epo R). The full length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) (a 24 aa hydrophobic signal sequence, a 226 aa extracellular domain, a 22 aa transmembrane domain and a 236 aa cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected in human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

1. Barber, D.L. and A.D. D'Andrea (1992) Seminars in Hematology 29:293.
2. Youssoufian, H. et al. (1993) Blood 9:2223.
3. Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
4. Baynes, R.D. et al. (1993) Blood 82:2088.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPOR
• Uniprot:
  • P19235()