Erythropoietin R Antibody (38409) [Fluorescein]

TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB307F, filled histogram) or isotype control antibody (IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

KU812 cells were stained with Mouse Anti-Human Erythropoietin R Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB307F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 38409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

Erythropoietin R Antibody (38409) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
  Pro26-Pro250
  Accession # P19235
• Specificity: Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Erythropoietin R is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPOR
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin R Antibody (38409) [Fluorescein]

• EpoR
• EPO-R
• Erythropoietin R
• erythropoietin receptor
• MGC138358

Background

Erythropoietin R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Erythropoietin and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Erythropoietin R Antibody (FAB307F)(5)

Publications using FAB307F

• Konstantin Musiychuk, Rajarajeswari Sivalenka, Jennifer Jaje, Hong Bi, Rosemary Flores, Brenden Shaw et al. Plant-Produced Human Recombinant Erythropoietic Growth Factors Support Erythroid Differentiation In Vitro Stem Cells and Development 8/15/2013 [PMID: 23517237]
• Peter Solár, Gabriela Hrčková, Lenka Varinská, Zuzana Solárová, Ján Kriška, Ivana Uhrínová et al. Location and the functionality of erythropoietin receptor(s) in A2780 cells Oncology Reports 4/30/2012 [PMID: 22552716]
• Peter Solár, Gabriela Hrčková, Lenka Varinská, Zuzana Solárová, Ján Kriška, Ivana Uhrínová et al. Location and the functionality of erythropoietin receptor(s) in A2780 cells Oncology Reports 2012-04-30 [PMID: 22552716] (Flow Cytometry, Human)
• Kessler C, Greindl A, Breuer B, Haberl U, Rybka A, Emgenbroich M, Frank HG, Potgens AJ Erythropoietin mimetic compound AGEM400(HES) binds to the same receptor as erythropoietin but displays a different spectrum of activities. Cytokine, 2011-12-03;57(2):226-37. 2011-12-03 [PMID: 22142701] (Flow Cytometry, Human)
• Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities. Blood, 2011-01-21;117(13):3485-93. 2011-01-21 [PMID: 21258008] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: EPOR
• Uniprot:
  • P19235()