Erythropoietin/EPO Antibody (AE7A5) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, AP
Clone
AE7A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Erythropoietin/EPO Antibody (AE7A5) [Unconjugated] Summary

Immunogen
Erythropoietin/EPO synthetic peptide analog
Ala28-Thr53
Accession # P01588
Specificity
Recognizes an epitope within the first 26 amino acids at the NH2 terminus of human urinary and human recombinant EPO. Binds human and rat EPO but does not bind sheep, baboon, or dog EPO (1, 2).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
EPO
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunoaffinity Purification
Publications
Read Publications using
MAB2871 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin/EPO Antibody (AE7A5) [Unconjugated]

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Erythropoietin/EPO Antibody (MAB2871)(8)

We have publications tested in 4 confirmed species: Human, Bovine, Chicken, Hamster.

We have publications tested in 2 applications: Functional Assay, Western Blot.


Filter By Application
Functional Assay
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(4)
Bovine
(1)
Chicken
(1)
Hamster
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB2871 Applications Species
CL Chin, JB Goh, H Srinivasan, KI Liu, A Gowher, R Shanmugam, HL Lim, M Choo, WQ Tang, AH Tan, T Nguyen-Khu, MH Tan, SK Ng A human expression system based on HEK293 for the stable production of recombinant erythropoietin Sci Rep, 2019;9(1):16768. 2019 [PMID: 31727983] (Western Blot, Human) Western Blot Human
MS Kwon, BC Koo, D Kim, YH Nam, XS Cui, NH Kim, T Kim Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites PLoS ONE, 2018;13(5):e0194721. 2018 [PMID: 29847554] (Western Blot, Chicken) Western Blot Chicken
de Lucas Cerrillo A, Bond W, Rex T Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin. Gene Ther, 2015;22(5):365-73. 2015 [PMID: 25716531] (Functional Assay, Hamster) Functional Assay Hamster
Park S, Won S, Song J, Kambe T, Nagao M, Kim W, Moon S EPO gene expression promotes proliferation, migration and invasion via the p38MAPK/AP-1/MMP-9 pathway by p21WAF1 expression in vascular smooth muscle cells. Cell Signal, 2015;27(3):470-8. 2015 [PMID: 25496832] (Western Blot, Human) Western Blot Human
Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R Novel human renal proximal tubular cell line for the production of complex proteins. J Biotechnol, 0;176(0):29-39. 0 [PMID: 24553072]
DeNiro M, Al-Mohanna F, Alsmadi O, Al-Mohanna F The nexus between VEGF and NFkappaB orchestrates a hypoxia-independent neovasculogenesis. PLoS ONE, 2013;8(3):e59021. 2013 [PMID: 23533599] (Western Blot, Human) Western Blot Human
Gassmann M, Grenacher B, Rohde B, Vogel J Quantifying Western blots: pitfalls of densitometry. Electrophoresis, 2009;30(11):1845-55. 2009 [PMID: 19517440] (Western Blot, Bovine) Western Blot Bovine
Delanghe JR, Bollen M, Beullens M Testing for recombinant erythropoietin. Am. J. Hematol., 2008;83(3):237-41. 2008 [PMID: 17918255] (Western Blot, Human) Western Blot Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Erythropoietin/EPO Antibody (MAB2871) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAM2871

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Bioinformatics Tool for Erythropoietin/EPO Antibody (MAB2871)

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Diseases for Erythropoietin/EPO Antibody (MAB2871)

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PTMs for Erythropoietin/EPO Antibody (MAB2871)

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Bioinformatics

Gene Symbol EPO
Uniprot