Erythropoietin/EPO Antibody (AE7A5) [Biotin]

• Reactivity: Hu
• Applications: WB
• Clone: AE7A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

Erythropoietin/EPO Antibody (AE7A5) [Biotin] Summary

• Immunogen: Erythropoietin/EPO synthetic peptide analog
  Ala28-Thr53
  Accession # P01588
• Specificity: Recognizes an epitope within the first 26 amino acids at the NH₂ terminus of human urinary and human recombinant EPO. Binds human and rat EPO but does not bind sheep, baboon, or dog EPO (1, 2).
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin/EPO Antibody (AE7A5) [Biotin]

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

EPO, a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

1. Sue and Sytkowski (1983) Proc. Natl. Acad. Sci. USA 80:3651.
2. Sytkowski and Fisher (1985) J. Biol. Chem. 260:14727.
3. Martinelli et al. (1985) Clin. Res. 33:349A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Erythropoietin/EPO Antibody (BAM2871)(1)

Publication using BAM2871

• Alexandre Marchand, Geoff Miller, Laurent Martin, Coralie Gobbo, Andre K. Crouch, Daniel Eichner et al. Detection of erythropoiesis stimulating agent Luspatercept after administration to healthy volunteers for antidoping purposes Drug Testing and Analysis 2022-11-01 [PMID: 35789123] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • P01588()