ERR beta/NR3B2 Antibody (H6705) [Unconjugated]

Immediate firing motoneurons, but not delayed firing motoneurons, express ERR beta .(A) Examples of Err beta . staining (red) in neurobiotin (green) filled motoneurons. The arrowhead in the first row points to the nucleus (Err beta −positive) of the recorded motoneuron. The asterisk in the second row indicates that the nucleus of the recorded motoneuron was Err beta −negative. (B) Plot of the AHP relaxation time constants against the rheobases for labelled motoneurons. All immediate firing motoneurons were Err beta −positive whereas all delayed firing motoneurons were Err beta −negative. Arrowheads point to the motoneurons illustrated in A.DOI://dx.doi.org/10.7554/eLife.04046.008 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/04046), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: H6705
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23015

ERR beta/NR3B2 Antibody (H6705) [Unconjugated] Summary

• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESRRB

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store the unopened product at -70 degreesC. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN 3 .
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERR beta/NR3B2 Antibody (H6705) [Unconjugated]

• ERR beta
• ERR2
• ESRL2
• ESRRB
• estrogen-related receptor beta
• NR3B2

Background

Estrogen-related receptor alpha (ERR-alpha, ERR1, ESRL1; NR3B1) is a member of the orphan nuclear receptor family. ERR-alpha is expressed in the liver, kidney, brain, heart, uterus, vagina and cervix. ERR-alpha takes part in the cell cycle and the fatty acid metabolizing of the musculus skeleti. ERR-alpha was shown to interact with ER-alpha. ERR-beta (ERR2; NR3B2) is also expressed in the liver, brain, uterus, vagina, and cervix. ERR-beta has a role in early placental development. ERR-gamma (ESRRG; NR3B3) is expressed in the brain, kidney, testis, lung, adrenal gland, pancreas, placenta and bone marrow. ERR-gamma was shown to be located in the critical region for Usher's syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERR beta/NR3B2 Antibody (PP-H6705-00)(26)

Publications using PP-H6705-00

• Li, QS;Zheng, PS; ESRRB inhibits the TGF-? signaling pathway to drive cell proliferation in cervical cancer Cancer research 2023-06-23 [PMID: 37350664] (IHC, Western Blot, ChIP, Human)
• Zhu, Z;Chen, X;Guo, A;Manzano, T;Walsh, PJ;Wills, KM;Halliburton, R;Radko-Juettner, S;Carter, RD;Partridge, JF;Green, DR;Zhang, J;Roberts, CWM; Mitotic bookmarking by SWI/SNF subunits Nature 2023-06-01 [PMID: 37225980] (Bioassay, ICC, Mouse)
• R Yelagandul, K Stecher, M Novatchkov, L Michetti, G Michlits, J Wang, P Hofbauer, G Vainorius, C Pribitzer, L Isbel, S Mendjan, D Schübeler, U Elling, J Brennecke, O Bell ZFP462 safeguards neural lineage specification by targeting G9A/GLP-mediated heterochromatin to silence enhancers Nature Cell Biology, 2023-01-05;0(0):. 2023-01-05 [PMID: 36604593] (ChIP, Mouse)
• MN Khan, P Cherukuri, F Negro, A Rajput, P Fabrowski, V Bansal, C Lancelin, TI Lee, Y Bian, WP Mayer, T Akay, D Müller, S Bonn, D Farina, T Marquardt ERR2 and ERR3 promote the development of gamma motor neuron functional properties required for proprioceptive movement control PloS Biology, 2022-12-21;20(12):e3001923. 2022-12-21 [PMID: 36542664] (IHC, Human)
• Sapir Herchcovici Levy, Sharon Feldman Cohen, Lee Arnon, Shlomtzion Lahav, Muhammad Awawdy, Adi Alajem et al. Esrrb is a cell-cycle-dependent associated factor balancing pluripotency and XEN differentiation Stem Cell Reports 2022-06-01 [PMID: 35594859]
• Daisuke Suzuki, Keitaro Okura, Seina Nagakura, Hidehiko Ogawa CDX2 downregulation in mouse mural trophectoderm during peri‐implantation is heteronomous, dependent on the YAP‐TEAD pathway and controlled by estrogen‐induced factors Reproductive Medicine and Biology 2022-02-27 [PMID: 35386376]
• Kang-Xuan Jin, Rujuan Zuo, Konstantinos Anastassiadis, Arne Klungland, Carsten Marr, Adam Filipczyk N6-methyladenosine (m 6 A) depletion regulates pluripotency exit by activating signaling pathways in embryonic stem cells Proceedings of the National Academy of Sciences 2021-12-21 [PMID: 34921114]
• J Bayerl, M Ayyash, T Shani, YS Manor, O Gafni, R Massarwa, Y Kalma, A Aguilera-C, M Zerbib, H Amir, D Sheban, S Geula, N Mor, L Weinberger, S Naveh Tass, V Krupalnik, B Oldak, N Livnat, S Tarazi, S Tawil, E Wildschutz, S Ashouokhi, L Lasman, V Rotter, S Hanna, D Ben-Yosef, N Novershter, S Viukov, JH Hanna Principles of signaling pathway modulation for enhancing human naive pluripotency induction Cell Stem Cell, 2021-04-28;0(0):. 2021-04-28 [PMID: 33915080] (ICC, Mouse)
• R Fan, YS Kim, J Wu, R Chen, D Zeuschner, K Mildner, K Adachi, G Wu, S Galatidou, J Li, HR Schöler, SA Leidel, I Bedzhov Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause Nat Commun, 2020-10-30;11(1):5499. 2020-10-30 [PMID: 33127892] (ICC, IHC, Mouse)
• Lior Lasman, Vladislav Krupalnik, Sergey Viukov, Nofar Mor, Alejandro Aguilera-Castrejon, Dan Schneir et al. Context-dependent functional compensation between Ythdf m6A reader proteins Genes & Development 2020-10-01 [PMID: 32943573]
• Aurélia Huyghe, Giacomo Furlan, Duygu Ozmadenci, Christina Galonska, Jocelyn Charlton, Xavier Gaume et al. Netrin-1 promotes naive pluripotency through Neo1 and Unc5b co-regulation of Wnt and MAPK signalling Nature Cell Biology 2020-04-01 [PMID: 32231305]
• Aileen I. Fernandez, Xue Geng, Krysta Chaldekas, Brent Harris, Anju Duttargi, V. Layne Berry et al. The orphan nuclear receptor estrogen-related receptor beta (ERR beta ) in triple-negative breast cancer Breast Cancer Research and Treatment 2020-02-01 [PMID: 31741180]
• Garland W, Comet I, Wu M et al. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2 Cell Rep 11/12/2019 [PMID: 31722198]
• A Bahat, A Goldman, Y Zaltsman, DH Khan, C Halperin, E Amzallag, V Krupalnik, M Mullokando, A Silberman, A Erez, AD Schimmer, JH Hanna, A Gross MTCH2-mediated mitochondrial fusion drives exit from na�ve pluripotency in embryonic stem cells Nat Commun, 2018-12-03;9(1):5132. 2018-12-03 [PMID: 30510213] (ICC, Human)
• JL Sardina, S Collombet, TV Tian, A Gómez, B Di Stefano, C Berenguer, J Brumbaugh, R Stadhouder, C Segura-Mor, M Gut, IG Gut, S Heath, S Aranda, L Di Croce, K Hochedling, D Thieffry, T Graf Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate Cell Stem Cell, 2018-09-13;0(0):. 2018-09-13 [PMID: 30220521] (Immunoprecipitation, Mouse)
• Amanda Andersson-Rolf, Roxana C. Mustata, Alessandra Merenda, Jihoon Kim, Sajith Perera, Tiago Grego et al. One-step generation of conditional and reversible gene knockouts Nature Methods 2017-03-01 [PMID: 28135257]
• Daniel A Rappolee Departure from optimal O2 level for mouse trophoblast stem cell proliferation and potency leads to most rapid AMPK activation J. Reprod. Dev., 2016-11-18;0(0):. 2016-11-18 [PMID: 27867161] (Western Blot, Mouse)
• Heckler MM, Zeleke TZ, Divekar SD et al. Antimitotic activity of DY131 and the estrogen-related receptor beta 2 (ERRb2) splice variant in breast cancer Oncotarget 7/26/2016 [PMID: 27363015]
• Yuan Lu, Jilong Li, Jianlin Cheng, Dennis B. Lubahn Messenger RNA profile analysis deciphers new Esrrb responsive genes in prostate cancer cells BMC Molecular Biology 2015-12-01 [PMID: 26627478]
• Yuan Lu, Jilong Li, Jianlin Cheng, Dennis B. Lubahn Genes targeted by the Hedgehog-signaling pathway can be regulated by Estrogen related receptor beta BMC Molecular Biology 2015-12-01 [PMID: 26597826]
• Mary Mazzotta Heckler, Rebecca B Riggins ERR beta splice variants differentially regulate cell cycle progression Cell Cycle 2015-01-02 [PMID: 25496115]
• Kumar , Roshan M, Cahan , Patrick, Shalek , Alex K, Satija , Rahul, DaleyKeyser , A Jay, Li , Hu, Zhang , Jin, Pardee , Keith, Gennert , David, Trombetta , John J, Ferrante , Thomas C, Regev , Aviv, Daley , George Q, Collins , James J Deconstructing transcriptional heterogeneity in pluripotent stem cells. Nature, 2014-12-04;516(7529):56-61. 2014-12-04 [PMID: 25471879] (IHC, Mouse)
• Félix Leroy, Boris Lamotte d'Incamps, Rebecca D Imhoff-Manuel, Daniel Zytnicki Early intrinsic hyperexcitability does not contribute to motoneuron degeneration in amyotrophic lateral sclerosis eLife 2014-10-14 [PMID: 25313866]
• Aliaksandra Radzisheuskaya, Gloryn Le Bin Le Bin Chia, Rodrigo L. dos Santos, Thorold W. Theunissen, L. Filipe C. Castro, Jennifer Nichols et al. A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages Nature Cell Biology 2013-06-01 [PMID: 23629142]
• Percharde M, Lavial F, Ng J, Kumar V, Tomaz R, Martin N, Yeo J, Gil J, Prabhakar S, Ng H, Parker M, Azuara V Ncoa3 functions as an essential Esrrb coactivator to sustain embryonic stem cell self-renewal and reprogramming. Genes Dev, 2012-09-26;26(20):2286-98. 2012-09-26 [PMID: 23019124] (ICC, Mouse)
• van den Berg DL, Zhang W, Yates A, Engelen E, Takacs K, Bezstarosti K, Demmers J, Chambers I, Poot RA Estrogen-related receptor beta interacts with Oct4 to positively regulate Nanog gene expression. Mol. Cell. Biol., 2008-07-28;28(19):5986-95. 2008-07-28 [PMID: 18662995] (ICC, Human)

Bioinformatics

• Gene Symbol: ESRRB